ArticleScientific reports2019
Combining ddPCR and environmental DNA to improve detection capabilities of a critically endangered freshwater invertebrate.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in Ghana.Molecular ecology resources · 2026Article
- From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.Molecular ecology resources · 2026Article
- One Assay to Rule Them All: Development of a Global Environmental DNA Tool to Support Range-Wide Surveys of an Imperiled Sawfish and Its Application in the Eastern Tropical Pacific.Ecology and evolution · 2026Article
- Unlocking Demography: Developing an eDNA-Based Toolkit to Measure Sex Ratios From Populations.Molecular ecology resources · 2026Article
- Rapid DNA/eDNA-Based ID Tools for Improved Chondrichthyan Monitoring and Management.Molecular ecology resources · 2025Review
- Comparing the precision of two digital PCR applications for copy number comparisons in protists.Scientific reports · 2025Article
- Environmental DNA and Hydroacoustic Surveys for Monitoring the Spread of the Invasive European Catfish (Animals : an open access journal from MDPI · 2025Article
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- Quantifying the Detection Sensitivity and Precision of qPCR and ddPCR Mechanisms for eDNA Samples.Ecology and evolution · 2024Article
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- Optimizing detectability of the endangered fan mussel using eDNA and ddPCR.Ecology and evolution · 2024Article
- Assessing the breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring.PeerJ · 2023Article
- Droplet digital PCR application for the detection of SARS-CoV-2 in air sample.Frontiers in public health · 2023Article
- Waterborne pathogens detection technologies: advances, challenges, and future perspectives.Frontiers in microbiology · 2023Review
- MULTI-STATE OCCUPANCY MODEL ESTIMATES PROBABILITY OF DETECTION OF AN AQUATIC PARASITE USING ENVIRONMENTAL DNA: PSEUDOLOMA NEUROPHILIA IN ZEBRAFISH AQUARIA.The Journal of parasitology · 2022Article
- A review of applications of environmental DNA for reptile conservation and management.Ecology and evolution · 2022Review
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
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- Persistence and accumulation of environmental DNA from an endangered dragonfly.Scientific reports · 2021Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Isogenus nubecula is a critically endangered Plecoptera species. Considered extinct in the UK, I. nubecula was recently rediscovered (in one location of the River Dee, Wales), after 22 years of absence. In a similar way to many other species of Perlodidae, I. nubecula could be utilised as a bio-indicator, for assessing water quality and health status of a given freshwater system. However, conventional monitoring of invertebrates via kick-sampling, is invasive and expensive (time consuming). Further, such methods require a high level of taxonomic expertise. Here, we compared the traditional kick-sampling method with the use of eDNA detection using qPCR and ddPCR-analyses. In spring 2018, we sampled eDNA from twelve locations on the River Dee. I. nubecula was detected using kick-sampling in five of these locations, three locations using both eDNA detection and kick-sampling and one location using eDNA detection alone - resulting in a total of six known and distinct populations of this critically endangered species. Interestingly, despite the eDNA assay being validated in vitro and in silico, and results indicating high sensitivity, qPCR analysis of the eDNA samples proved to be ineffective. In contrast, ddPCR analyses resulted in a clear detection of I. nubecula at four locations suggesting that inhibition most likely explains the large discrepancy between the obtained qPCR and ddPCR results. It is therefore important to explore inhibition effects on any new eDNA assay. We also highlight that ddPCR may well be the best option for the detection of aquatic organisms which are either rare or likely to shed low levels of eDNA into their environment.
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