ArticleProceedings of the National Academy of Sciences of the United States of America2019
Reviving rare chicken breeds using genetically engineered sterility in surrogate host birds.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 83 citations in OpenAlex.
- DAZL-targeted inducible kill switches enable efficient ablation of chicken primordial germ cells.Poultry science · 2026Article
- Review
- Transcriptional Profiling of Primordial Germ Cells During Chicken Embryonic Development.Veterinary sciences · 2026Article
- NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis.Poultry science · 2026Article
- Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing.Development, growth & differentiation · 2026Article
- In ovo sexing and genotyping using PCR techniques: a contribution to the 3R principles in chicken breeding.Scientific reports · 2026Article
- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- Establishment, Characterization, and Cryopreservation of Feather Follicle Fibroblast Lines From Hyacinth Macaw (Anodorhynchus hyacinthinus).Cell biology international · 2025Article
- A practical approach for the stable isolation and cultivation of chicken gonadal primordial germ cells with mitotically inactivated STO feeder cells.Animal bioscience · 2025Article
- In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.Biology · 2025Review
- Capturing avian somatic cells using feather pulp fibroblast culture as a non-invasive approach to biobanking endangered birds.Cytotechnology · 2025Article
- Functional Equivalence of Insulin and IGF-1 in the In Vitro Culture of Chicken Primordial Germ Cells.Genes · 2025Article
- Chicken Primordial Germ Cells Do Not Proliferate in Insulin-Lacking Media.International journal of molecular sciences · 2025Article
- Propagation of goose primordial germ cells in vitro relies on FGF and BMP signalling pathways.Communications biology · 2025Article
- Achieving Optimal Transfection Conditions in Chicken Primordial Germ Cells Under Feeder- and Serum-Free Medium.Animals : an open access journal from MDPI · 2025Article
- Comparison of primordial germ cell differences at different developmental time points in chickens.Animal bioscience · 2024Article
- PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.Communications biology · 2024Article
- Article
- Successful xenotransplantation of testicular cells following fractionated chemotherapy of recipient birds.Scientific reports · 2024Article
- Avian iPSC Derivation to Recover Threatened Wild Species: A Comprehensive Review in Light of Well-Established Protocols.Animals : an open access journal from MDPI · 2024Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
In macrolecithal species, cryopreservation of the oocyte and zygote is not possible due to the large size and quantity of lipid deposited within the egg. For birds, this signifies that cryopreserving and regenerating a species from frozen cellular material are currently technically unfeasible. Diploid primordial germ cells (PGCs) are a potential means to freeze down the entire genome and reconstitute an avian species from frozen material. Here, we examine the use of genetically engineered (GE) sterile female layer chicken as surrogate hosts for the transplantation of cryopreserved avian PGCs from rare heritage breeds of chicken. We first amplified PGC numbers in culture before cryopreservation and subsequent transplantation into host GE embryos. We found that all hatched offspring from the chimera GE hens were derived from the donor rare heritage breed broiler PGCs, and using cryopreserved semen, we were able to produce pure offspring. Measurement of the mutation rate of PGCs in culture revealed that 2.7 × 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.