ArticleInternational journal of molecular sciences2019
Characterization and Establishment of an Immortalized Rabbit Melanocyte Cell Line Using the SV40 Large T Antigen.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
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- Establishment and evaluation of an immortalized dzo kidney cell line.Scientific reports · 2025Article
- Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability.Animal models and experimental medicine · 2024Article
- Characterization and arbovirus susceptibility of cultured CERNI cells derived from sika deer (Cervus nippon).In vitro cellular & developmental biology. Animal · 2024Article
- Characterization and establishment of an immortalized rabbit ovary granulosa cell line for reproductive biology experiments.In vitro cellular & developmental biology. Animal · 2024Article
- Establishment and functional characterization of immortalized rabbit dermal papilla cell lines.Animal biotechnology · 2023Article
- Construction and Characterization of Immortalized Fibroblast Cell Line from Bactrian Camel.Life (Basel, Switzerland) · 2023Article
- Establishment and characterization of an immortalized bovine intestinal epithelial cell line.Journal of animal science · 2023Article
- Generation and characterization of two immortalized dermal fibroblast cell lines from the spiny mouse (Acomys).PloS one · 2023Article
- Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to HMolecules (Basel, Switzerland) · 2022Article
- Generation and characterization of human Fetal membrane and Decidual cell lines for reproductive biology experiments†.Biology of reproduction · 2022Article
- Establishment methods and research progress of livestock and poultry immortalized cell lines: A review.Frontiers in veterinary science · 2022Review
- Immunostimulatory Activity of Synbiotics UsingMicroorganisms · 2021Article
- Establishment and characterization of an immortalized epicardial cell line.Journal of cellular and molecular medicine · 2021Article
- Characterization ofGenes · 2020Article
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- Transgenic Immortalization of Human Dermal Fibroblasts Mediated Through the MicroRNA/SIRT1 Pathway.In vivo (Athens, Greece)Article
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Authors and funding
11 authors at 2 institutions in 1 country.
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Abstract
Melanocytes (MCs) are specialized cells that synthesize melanin within the melanosome. Cultured MCs are useful in order to study their role in relation to pigmentation. However, MC isolation is laborious and the obtained cells have a limited culture time. In this study, we transformed lentivirus-mediated simian virus 40 Large T (SV40-LT) into primary rabbit melanocytes (Pri RMCs) to establish an immortalized cell line. Morphologically, the immortalized RMCs (Im RMC) were indistinguishable from the Pri RMCs, and dendrites were visible following Dopa staining. No significant differences in cell proliferation or growth between immortalized and primary RMCs were observed. Based on melanocyte-specific markers, the expression of MITF, TYR, and TYRP1 were detected by PCR, immunofluorescence staining, and western blot analysis. Through karyotype, soft agar, and tumorigenesis assays, the immortalized RMCs did not undergo malignant transformation. Our results show that Im RMCs can be used as a tool cell for future MC studies on the pigmentation mechanisms of fur animals.
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