ArticlePlants (Basel, Switzerland)2019
Efficient Biolistic Transformation of Immature Citrus Rootstocks Using Phosphomannose-isomerase Selection.
Article in Plants (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.International journal of molecular sciences · 2024Review
- Transient expression of anti-HrpE scFv antibody reduces the hypersensitive response in non-host plant against bacterial phytopathogen Xanthomonas citri subsp. citri.Scientific reports · 2024Article
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
- Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant.Plant cell reports · 2021Article
- Compendium of Plant-Specific CRISPR Vectors and Their Technical Advantages.Life (Basel, Switzerland) · 2021Review
- A simple and efficient agroinfiltration method for transient gene expression in Citrus.Plant cell reports · 2021Article
- An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue.Scientific reports · 2021Article
- Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato.Plant diversity · 2021Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- Article
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- Citrus Taste Modification Potentials by Genetic Engineering.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
This research utilized the E. coli manA gene encoding phosphomannose isomerase (PMI) selection on sucrose/mannose medium to increase transformation efficiencies after biolistic transformation of two immature citrus rootstock cultivars. Plasmid DNA, containing the manA gene and the enhanced green fluorescent protein (egfp) reporter gene, was bombarded into epicotyl explants of immature Carrizo citrange and Swingle citrumelo. GFP positive shoots were micro-grafted onto in vitro grown immature Carrizo rootstocks. Nineteen transgenic Carrizo shoots were obtained from ten paired shots, and eight Swingle shoots from five paired shots. The mean transformation efficiency of Carrizo was 1.9 transgenics/paired shot while the transformation efficiency of Swingle was comparable at 1.6 transgenics/paired shot. The transformants were analyzed by PCR for the presence of transgenes. Southern blot analysis of eight representative Carrizo transgenic events and four Swingle transgenic events showed that all transgenics had one to three copies of the manA gene. The PMI enzyme activity in the transgenic lines was confirmed using the chlorophenol red assay.
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Registered trials
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