ArticleJOR spine2019
Radial variation in biochemical composition of the bovine caudal intervertebral disc.
Article in JOR spine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Physeal effects of posterior VBT are not uniform throughout a multi-tether construct in the kyphotic swine model.Spine deformity · 2026Article
- The effect of spaceflight on tissues of the spinal column.Bone reports · 2026Review
- Complex Mechanical Loading and Pro-Inflammatory Cytokines in Intervertebral Disc Degeneration.JOR spine · 2026Article
- The growth modulating effects of tether tension on vertebral growth are biphasic: a study of posterior vertebral body tethering (pVBT) in a novel kyphotic porcine model.Spine deformity · 2026Article
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- Effects of high-altitude environments on intervertebral disc degeneration and transcriptome profiling of the nucleus pulposus.Frontiers in cell and developmental biology · 2025Article
- Understanding the etiopathogenesis of lumbar intervertebral disc herniation: From clinical evidence to basic scientific research.JOR spine · 2024Review
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- Annular Defects Impair the Mechanical Stability of the Intervertebral Disc.Global spine journal · 2023Article
- Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures.International journal of molecular sciences · 2023Article
- Design of a flexing organ-chip to modelBiomicrofluidics · 2022Article
- Single-cell RNA-sequencing atlas of bovine caudal intervertebral discs: Discovery of heterogeneous cell populations with distinct roles in homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Multiaxial validation of a finite element model of the intervertebral disc with multigenerational fibers to establish residual strain.JOR spine · 2021Article
- One strike loading organ culture model to investigate the post-traumatic disc degenerative condition.Journal of orthopaedic translation · 2021Article
- A Robust Multiscale and Multiphasic Structure-Based Modeling Framework for the Intervertebral Disc.Frontiers in bioengineering and biotechnology · 2021Article
- Spatial mapping of collagen content and structure in human intervertebral disk degeneration.JOR spine · 2020Article
- Article
- Imaging the local biochemical content of native and injured intervertebral disc using Fourier transform infrared microscopy.JOR spine · 2020Article
- Radial variation in biochemical composition of the bovine caudal intervertebral disc.JOR spine · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine caudal discs have been widely used in spine research due to their increased availability, large size, and mechanical and biochemical properties that are comparable to healthy human discs. However, despite their extensive use, the radial variations in bovine disc composition have not yet been rigorously quantified with high spatial resolution. Previous studies were limited to qualitative analyses or provided limited spatial resolution in biochemical properties. Thus, the main objective of this study was to provide quantitative measurements of biochemical composition with higher spatial resolution than previous studies that employed traditional biochemical techniques. Specifically, traditional biochemical analyses were used to measure water, sulfated glycosaminoglycan, collagen, and DNA contents. Gravimetric water content was compared to data obtained through Raman spectroscopy and differential scanning calorimetry. Additionally, spatial distribution of lipids in the disc's collagen network was visualized and quantified, for the first time, using multi-modal second harmonic generation (SHG) and Coherent anti-Stokes Raman (CARS) microscopy. Some heterogeneity was observed in the nucleus pulposus, where the water content and water-to-protein ratio of the inner nucleus were greater than the outer nucleus. In contrast, the bovine annulus fibrosus exhibited a more heterogeneous distribution of biochemical properties. Comparable results between orthohydroxyproline assay and SHG imaging highlight the potential benefit of using SHG microscopy as a less destructive method for measuring collagen content, particularly when relative changes are of interest. CARS images showed that lipid deposits were distributed equally throughout the disc and appeared either as individual droplets or as clusters of small droplets. In conclusion, this study provided a more comprehensive assessment of spatial variations in biochemical composition of the bovine caudal disc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.