Evidence map›Paper›PMID 31572547›Full record

ArticleExperimental and therapeutic medicine2019

MicroRNA-146a inhibits NF-κB activation and pro-inflammatory cytokine production by regulating IRAK1 expression in THP-1 cells.

Chunlei Zhou, Lan Zhao, Kai Wang, Qianru Qi, Meng Wang, Lei Yang, Ping Sun, Hong Mu

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed, 3 pooled it
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 3 syntheses or guidelines pooled it, 61 citations in OpenAlex.

  1. Pooled it
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  3. Genetic variant inAnnals of medicine · 2021
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  19. Therapeutic Applications of Poly-miRNAs and miRNA Sponges.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Chunlei ZhouDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Lan ZhaoLaboratory of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China.
Kai WangDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Qianru QiDepartment of Medical Laboratory, Tianjin Children's Hospital, Tianjin 300134, P.R. China.
Meng WangDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Lei YangDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Ping SunDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Hong MuDepartment of Medical Laboratory, Tianjin First Central Hospital, Tianjin 300192, P.R. China.
Tianjin First Center Hospital · CNTianjin Medical University · CNFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine · CNTianjin Children's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNA (miR)-146a levels are reduced in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE); however, its function is not well understood. The present study investigated the role of miR-146a in the regulation of lipopolysaccharide (LPS)-induced inflammation in THP-1 cells. A miR-146a mimic and an inhibitor were used to overexpress and downregulate miR-146a expression, respectively. Reverse transcription-quantitative PCR and western blot analyses were performed to evaluate interleukin (IL)-1 receptor-associated kinase 1 (IRAK1) expression, and western blot analysis was applied to assess nuclear factor-κB activation by analyzing p65 subunit levels in the nucleus. To investigate the effects of miR-146a on LPS-induced inflammation, IL-6 and tumor necrosis factor-α (TNF-α) levels were also measured using ELISA. The results of the present study revealed thatmiR-146a overexpression significantly reduced IRAK1 expression, reduced p65 levels in the nucleus and reduced IL-6 and TNF-α levels in the supernatant of the cell culture medium of THP-1 cells following LPS treatment. Luciferase assays confirmed IRAK1 to be a direct target of miR-146a in THP-1 cells. In conclusion, miR-146a may regulate IRAK1 expression and inhibit the activation of inflammatory signals and secretion of pro-inflammatory cytokines. The present study revealed, at least in part, the mechanisms by which miR-146a regulate the inflammatory response in SLE.

Indexed as

interleukin-1 receptor-associated kinase 1interleukin-6microRNA-146anuclear factor-κBsystemic lupus erythematosustumor necrosis factor-α

Identifiers

PMID31572547
PMCPMC6755493
OpenAlexW2968178830

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.