ArticleInternational journal of nanomedicine2019
Micropatterned nanolayers immobilized with nerve growth factor for neurite formation of PC12 cells.
Article in International journal of nanomedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Decreasing the physical gap in the neural-electrode interface and related concepts to improve cochlear implant performance.Frontiers in neuroscience · 2024Review
- Article
- Highlights on Advancing Frontiers in Tissue Engineering.Tissue engineering. Part B, Reviews · 2022Review
- Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate.Gels (Basel, Switzerland) · 2022Article
- Anisometric Microstructures to Determine Minimal Critical Physical Cues Required for Neurite Alignment.Advanced healthcare materials · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNerve regeneration is important for the treatment of degenerative diseases and neurons injured by accidents. Nerve growth factor (NGF) has been previously conjugated to materials for promotion of neurogenesis. MATERIALS AND
methodsPhotoreactive gelatin was prepared by chemical coupling of gelatin with azidobenzoic acid (P-gel), and then NGF was immobilized on substrates in the presence or absence of micropatterned photomasks. UV irradiation induced crosslinking reactions of P-gel with itself, NGF, and the plate for immobilization.
resultsBy adjustment of the P-gel concentration, the nanometer-order height of micropatterns was controlled. NGF was quantitatively immobilized with increasing amounts of P-gel. Immobilized NGF induced neurite outgrowth of PC12 cells, a cell line derived from a pheochromocytoma of the rat adrenal medulla, at the same level as soluble NGF. The immobilized NGF showed higher thermal stability than the soluble NGF and was repeatedly used without loss of biological activity. The 3D structure (height of the formed micropattern) regulated the behavior of neurite guidance. As a result, the orientation of neurites was regulated by the stripe pattern width.
conclusionThe micropattern-immobilized NGF nanolayer biochemically and topologically regulated neurite formation.
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