ArticleNature microbiology2019
Vibrio cholerae residing in food vacuoles expelled by protozoa are more infectious in vivo.
Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
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- Paramoeba atlantica as a Natural Intracellular Niche for Vibrios in Marine Ecosystems.Environmental microbiology · 2025Article
- Development of flow cytometry assay to quantify packaging of C. jejuni by Tetrahymena species.FEMS microbiology letters · 2025Article
- Tetrahymena predation drives adaptive evolution of Salmonella by disrupting O-antigen biosynthesis and upregulating transcriptional regulator csgD.The ISME journal · 2025Article
- Protozoa-enhanced conjugation frequency alters the dissemination of soil antibiotic resistance.The ISME journal · 2025Article
- Single-cell RNA-seq reveals a key role forFrontiers in microbiology · 2025Article
- Chromosomal integrons are genetically and functionally isolated units of genomes.Nucleic acids research · 2024Article
- Review
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- Protozoan predation enhances stress resistance and antibiotic tolerance in Burkholderia cenocepacia by triggering the SOS response.The ISME journal · 2024Article
- The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model.PLoS neglected tropical diseases · 2024Article
- Zooming in on the intracellular microbiome composition of bacterivorousISME communications · 2024Article
- Increased iron utilization and oxidative stress tolerance in aApplied and environmental microbiology · 2023Article
- Vibrio cholerae-An emerging pathogen in Austrian bathing waters?Wiener klinische Wochenschrift · 2023Review
- Phagotrophic protists preserve antibiotic-resistant opportunistic human pathogens in the vegetable phyllosphere.ISME communications · 2023Article
- Vibrio cholerae Invasion Dynamics of the Chironomid Host Are Strongly Influenced by Aquatic Cell Density and Can Vary by Strain.Microbiology spectrum · 2023Article
- Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.Advances in experimental medicine and biology · 2023Article
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14 authors at 3 institutions in 5 countries.
Funding
Abstract
Vibrio cholerae interacts with many organisms in the environment, including heterotrophic protists (protozoa). Several species of protozoa have been reported to release undigested bacteria in expelled food vacuoles (EFVs) when feeding on some pathogens. While the production of EFVs has been reported, their biological role as a vector for the transmission of pathogens remains unknown. Here we report that ciliated protozoa release EFVs containing V. cholerae. The EFVs are stable, the cells inside them are protected from multiple stresses, and large numbers of cells escape when incubated at 37 °C or in the presence of nutrients. We show that OmpU, a major outer membrane protein positively regulated by ToxR, has a role in the production of EFVs. Notably, cells released from EFVs have growth and colonization advantages over planktonic cells both in vitro and in vivo. Our results suggest that EFVs facilitate V. cholerae survival in the environment, enhancing their infectious potential and may contribute to the dissemination of epidemic V. cholerae strains. These results improve our understanding of the mechanisms of persistence and the modes of transmission of V. cholerae and may further apply to other opportunistic pathogens that have been shown to be released by protists in EFVs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.