ArticleScientific reports2019
Predicting DNA damage foci and their experimental readout with 2D microscopy: a unified approach applied to photon and neutron exposures.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Comprehensive database of track-structure simulations on DNA damage by H-Fe ions up to 1 GeV/u for space radiation biology.Scientific reports · 2026Article
- Temporal and spatial patterns of γH2AX signaling in different human cells after exposure to X-rays and UV-C light.Radiation and environmental biophysics · 2026Article
- Molecular and Cellular Effects of CT Scans in Human Adipose Mesenchymal Stem Cells.International journal of molecular sciences · 2025Article
- Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence.International journal of molecular sciences · 2024Article
- Single-molecule approaches for DNA damage detection and repair: A focus on Repair Assisted Damage Detection (RADD).DNA repair · 2023Review
- Review
- Computational modelling of γ-H2AX foci formation in human cells induced by alpha particle exposure.Scientific reports · 2022Article
- Dosimetry of heavy ion exposure to human cells using nanoscopic imaging of double strand break repair protein clusters.Scientific reports · 2022Article
- Medical application of particle and heavy ion transport code system PHITS.Radiological physics and technology · 2021Review
- Mechanistic Modelling of Slow and Fast NHEJ DNA Repair Pathways Following Radiation for G0/G1 Normal Tissue Cells.Cancers · 2021Article
- Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA.Scientific reports · 2020Article
- Analytical formulas representing track-structure simulations on DNA damage induced by protons and light ions at radiotherapy-relevant energies.Scientific reports · 2020Article
- Iron Ladies - How Desiccated Asexual RotiferFrontiers in microbiology · 2020Article
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Authors and funding
9 authors.
Funding
Abstract
The consideration of how a given technique affects results of experimental measurements is a must to achieve correct data interpretation. This might be challenging when it comes to measurements on biological systems, where it is unrealistic to have full control (e.g. through a software replica) of all steps in the measurement chain. In this work we address how the effectiveness of different radiation qualities in inducing biological damage can be assessed measuring DNA damage foci yields, only provided that artefacts related to the scoring technique are adequately considered. To this aim, we developed a unified stochastic modelling approach that, starting from radiation tracks, predicts both the induction, spatial distribution and complexity of DNA damage, and the experimental readout of foci when immunocytochemistry coupled to 2D fluorescence microscopy is used. The approach is used to interpret γ-H2AX data for photon and neutron exposures. When foci are reconstructed in the whole cell nucleus, we obtain information on damage characteristics "behind" experimental observations, as the average damage content of a focus. We reproduce how the detection technique affects experimental findings, e.g. contributing to the saturation of foci yields scored at 30 minutes after exposure with increasing dose and to the lack of dose dependence for yields at 24 hours.
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