ArticleScientific reports2019
Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- A high-yield, SPE-free DNA extraction method using a pyridinium-based ionic liquid compatible with downstream PCR analysis.Analytical and bioanalytical chemistry · 2026Article
- Restoration of β-lactam activity by nacubactam combinations against extensively drug-resistant K. pneumoniae harbouring diverse carbapenem resistance gene.World journal of microbiology & biotechnology · 2026Article
- Ionic liquid-assisted seed genomic DNA extraction for advanced sequencing applications.Plant methods · 2025Article
- A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics.Analytical and bioanalytical chemistry · 2024Article
- An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics.Biosensors · 2024Article
- Association of coagulase-negative staphylococci with orthopedic infections detected by in-house multiplex real-time PCR.Frontiers in microbiology · 2024Article
- The methane-oxidizing microbial communities of three maar lakes in tropical monsoon Asia.Frontiers in microbiology · 2024Article
- Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands.ACS applied materials & interfaces · 2023Article
- An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs.Microbial genomics · 2023Article
- Human vaginal microbiota colonization is regulated by female sex hormones in a mouse model.Frontiers in cellular and infection microbiology · 2023Article
- Shewanella jiangmenensis sp. nov., isolated from aquaculture water.Archives of microbiology · 2022Article
- Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies.Journal of animal science · 2022Review
- Improved DNA extraction on bamboo paper and cotton is tightly correlated with their crystallinity and hygroscopicity.PloS one · 2022Article
- Gut probiotic Lactobacillus rhamnosus attenuates PDE4B-mediated interleukin-6 induced by SARS-CoV-2 membrane glycoprotein.The Journal of nutritional biochemistry · 2021Article
- Novel Carbapenem-ResistantInfection and drug resistance · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The extraction of nucleic acids from microorganisms for subsequent molecular diagnostic applications is still a tedious and time-consuming procedure. We developed a method for the rapid preparation of genomic DNA from bacteria based on hydrophilic ionic liquids (ILs). First, we tested eight ILs in different buffer systems for their inhibitory effects on quantitative PCR. The cell lysis potential of different IL/buffer combinations was assessed by application on Enterococcus faecalis as a model organism for Gram-positive bacteria. The two best ILs, choline hexanoate and 1-ethyl-3-methylimidazolium acetate, were compared with the reference enzymatic method and two commercial DNA extraction kits. All methods were evaluated on four Gram-positive and four Gram-negative bacterial species that are highly relevant for environmental, food, or clinical diagnostics. In comparison to the reference method, extraction yields of the IL-based procedure were within one order of magnitude for most of the strains. The final protocol for DNA extraction using the two ILs is very low-cost, avoids the use of hazardous chemicals and can be performed in five minutes on a simple heating block. This makes the method ideal for high sample throughput and offers the opportunity for DNA extraction from bacteria in resource-limited settings or even in the field.
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