ArticleProceedings of the National Academy of Sciences of the United States of America2019
Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 0 citations in OpenAlex.
- Article
- Article
- Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of infection-critical peptide externalization.Biophysical journal · 2026Article
- Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.Annual review of biochemistry · 2026Review
- Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.European biophysics journal : EBJ · 2026Review
- Modeling Reveals How Direct-Acting Antivirals Redirect HBV Capsid Assembly Pathways to Noninfectious Products.bioRxiv : the preprint server for biology · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Screening metatranscriptomes for ultrastable RNA secondary structures reveals hidden bacteriophages and novel capsid nanomaterials.bioRxiv : the preprint server for biology · 2026Article
- Process for Standardizing and Assessing the Parameters Governing MS2 Virus-Like Particle Reassembly around Nucleic Acid Cargo.bioRxiv : the preprint server for biology · 2026Article
- From toroids to helical tubules: Kirigami-inspired programmable assembly of two-periodic curved crystals from DNA origami.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Extracellular uncoating of bacteriophage MS2.Journal of molecular biology · 2025Article
- Distinct Dimerization Mechanisms in Silkworm and Spider Silk Proteins Revealed by Mass Photometry.The journal of physical chemistry letters · 2025Article
- Measuring the selective packaging of RNA molecules by viral coat proteins in cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Protein-RNA condensation kinetics via filamentous nanoclusters.Protein science : a publication of the Protein Society · 2025Article
- Kinetic Description of Viral Capsid Self-Assembly Using Mesoscopic Non-Equilibrium Thermodynamics.Entropy (Basel, Switzerland) · 2025Article
- Disassembly of Virus-Like Particles and the Stabilizing Role of the Nucleic Acid Cargo.The journal of physical chemistry. B · 2025Article
- Stimulus-responsive assembly of nonviral nucleocapsids.Nature communications · 2024Article
- Synthesis, insertion, and characterization of SARS-CoV-2 membrane protein within lipid bilayers.Science advances · 2024Article
- Article
- Effect of coat-protein concentration on the self-assembly of bacteriophage MS2 capsids around RNA.Nanoscale · 2024Article
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3 authors at 1 institution in 1 country.
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Abstract
Self-assembly is widely used by biological systems to build functional nanostructures, such as the protein capsids of RNA viruses. But because assembly is a collective phenomenon involving many weakly interacting subunits and a broad range of timescales, measurements of the assembly pathways have been elusive. We use interferometric scattering microscopy to measure the assembly kinetics of individual MS2 bacteriophage capsids around MS2 RNA. By recording how many coat proteins bind to each of many individual RNA strands, we find that assembly proceeds by nucleation followed by monotonic growth. Our measurements reveal the assembly pathways in quantitative detail and also show their failure modes. We use these results to critically examine models of the assembly process.
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