ArticleMetabolites2019
Influence of Storage Conditions and Preservatives on Metabolite Fingerprints in Urine.
Article in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Daily Vinegar Ingestion Improves Depression Scores and Alters the Metabolome in Healthy Adults: A Randomized Controlled Trial.Nutrients · 2021Trial
- Effect of temperature and preservative selection on C-peptide degradation in randomly voided urine.Journal of clinical & translational endocrinology · 2026Article
- Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives.International urogynecology journal · 2025Article
- Influence of different urine preservation methods and addition of preservatives on quantitative detection of 24-h urinary protein.Frontiers in medicine · 2025Article
- Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine Biospecimens.medRxiv : the preprint server for health sciences · 2024Article
- UAS™-A Urine Preservative for Oncology Applications.Cancers · 2023Article
- Urinary Sucrose and Fructose From Spot Urine May Be Used as a Predictive Biomarker of Total Sugar Intake-Findings From a Controlled Feeding Study.The Journal of nutrition · 2023Article
- Impact of preanalytical freezing delay time on the stability of metabolites in oral squamous cell carcinoma tissue samples.Metabolomics : Official journal of the Metabolomic Society · 2022Article
- Article
- The circadian rhythm of calcium and bone homeostasis in Maasai.American journal of human biology : the official journal of the Human Biology Council · 2022Article
- Article
- Stability of novel urinary biomarkers used for lupus nephritis.Frontiers in pediatrics · 2022Article
- Nutritional Metabolomics and the Classification of Dietary Biomarker Candidates: A Critical Review.Advances in nutrition (Bethesda, Md.) · 2021Review
- From bedside to bench-practical considerations to avoid pre-analytical pitfalls and assess sample quality for high-resolution metabolomics and lipidomics analyses of body fluids.Analytical and bioanalytical chemistry · 2021Review
- Investigating the performance of 24-h urinary sucrose and fructose as a biomarker of total sugars intake in US participants - a controlled feeding study.The American journal of clinical nutrition · 2021Article
- Enhanced Detection of Short-Chain Fatty Acids Using Gas Chromatography Mass Spectrometry.Current protocols · 2021Article
- Standardization of microbiome studies for urolithiasis: an international consensus agreement.Nature reviews. Urology · 2021Article
- Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics.Metabolites · 2020Review
- A review of strategies for untargeted urinary metabolomic analysis using gas chromatography-mass spectrometry.Metabolomics : Official journal of the Metabolomic Society · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.