Evidence map›Paper›PMID 31569579›Full record

ReviewMicroorganisms2019

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.

Grant A R Gale, Alejandra A Schiavon Osorio, Lauren A Mills, Baojun Wang, David J Lea-Smith, Alistair J McCormick

Open access · goldAbstract readReview
In one paragraph

Review in Microorganisms, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
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  4. Article
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  8. Review
  9. Review
  10. Article
  11. Review
  12. Optogenetic control of gene expression in the cyanobacteriumFrontiers in bioengineering and biotechnology · 2024
    Article
  13. Review
  14. Temperature-induced zeaxanthin overproduction in Synechococcus elongatus PCC 7942.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023
    Article
  15. Review
  16. The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023
    Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Grant A R GaleInstitute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK. grant.gale@ed.ac.uk.
Alejandra A Schiavon OsorioInstitute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK. alejandra.schiavon@ed.ac.uk.
Lauren A MillsSchool of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. l.mills@uea.ac.uk.ORCID 0000-0002-7461-4182
Baojun WangCentre for Synthetic and Systems Biology, University of Edinburgh, Edinburgh EH9 3BF, UK. baojun.wang@ed.ac.uk.ORCID 0000-0002-4858-8937
David J Lea-SmithSchool of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. d.lea-smith@uea.ac.uk.
Alistair J McCormickInstitute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK. alistair.mccormick@ed.ac.uk.ORCID 0000-0002-7255-872X
University of Edinburgh · GBUniversity of East Anglia · GB

Funding

Medical Research Council MR/S018875/1
6 · The paper itself

Abstract

Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research. Nevertheless, work to date has focused primarily on only a small subset of model species, which arguably limits fundamental discovery and applied research towards wider commercialisation. Here, we review the requirements for uptake of new strains, including several recently characterised fast-growing species and promising non-model species. Furthermore, we discuss the potential applications of new techniques available for transformation, genetic engineering and regulation, including an up-to-date appraisal of current Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas) and CRISPR interference (CRISPRi) research in cyanobacteria. We also provide an overview of several exciting molecular tools that could be ported to cyanobacteria for more advanced metabolic engineering approaches (e.g., genetic circuit design). Lastly, we introduce a forthcoming mutant library for the model species

Indexed as

CRISPR/CasCRISPRigenetic circuitsgenome engineeringgenome-scale modelsinteinsmutant libraryoptogeneticsserine integrasesigma factorssynthetic biology

Identifiers

PMID31569579
PMCPMC6843473
OpenAlexW2977031274

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.