ArticlePLoS biology2019
EXP1 is critical for nutrient uptake across the parasitophorous vacuole membrane of malaria parasites.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 77 citations in OpenAlex.
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- Revisiting Glutathione Transferases ofPharmaceuticals (Basel, Switzerland) · 2026Review
- The parasitophorous vacuole membrane of Toxoplasma gondii: A custom structural framing with smart interior plumbing for resource allocation.PLoS pathogens · 2026Review
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Method for Purification and Electrophysiological Recordings of Ion Channels from Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nucleotide-resolution Mapping of RNA N6-Methyladenosine (m6A) modifications and comprehensive analysis of global polyadenylation events in mRNA 3' end processing in malaria pathogenbioRxiv : the preprint server for biology · 2025Article
- Adaptations and metabolic evolution of myzozoan protists across diverse lifestyles and environments.Microbiology and molecular biology reviews : MMBR · 2024Review
- APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection.PLoS pathogens · 2024Article
- Giant membrane rings/loops in the cytosol of P. falciparum-infected erythrocytes and their relation to the parasite.Parasitology research · 2024Article
- Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis.Nature communications · 2024Article
- Flp/Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Let it glow: genetically encoded fluorescent reporters in Plasmodium.Malaria journal · 2024Review
- Novel Ion Channel Genes in Malaria Parasites.Genes · 2024Review
- The Kelch13 compartment contains highly divergent vesicle trafficking proteins in malaria parasites.PLoS pathogens · 2023Article
- Critical interdependencies between Plasmodium nutrient flux and drugs.Trends in parasitology · 2023Review
- Knock-sideways by inducible ER retrieval enables a unique approach for studyingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Timing of dense granule biogenesis in asexual malaria parasites.Microbiology (Reading, England) · 2023Article
- PTEX helps efficiently traffic haemoglobinases to the food vacuole in Plasmodium falciparum.PLoS pathogens · 2023Article
- Genome-wide CRISPR screen identifies genes synthetically lethal with GRA17, a nutrient channel encoding gene in Toxoplasma.PLoS pathogens · 2023Article
- Roles of the RON3 C-terminal fragment in erythrocyte invasion and blood-stage parasite proliferation inFrontiers in cellular and infection microbiology · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular malaria parasites grow in a vacuole delimited by the parasitophorous vacuolar membrane (PVM). This membrane fulfils critical roles for survival of the parasite in its intracellular niche such as in protein export and nutrient acquisition. Using a conditional knockout (KO), we here demonstrate that the abundant integral PVM protein exported protein 1 (EXP1) is essential for parasite survival but that this is independent of its previously postulated function as a glutathione S-transferase (GST). Patch-clamp experiments indicated that EXP1 is critical for the nutrient-permeable channel activity at the PVM. Loss of EXP1 abolished the correct localisation of EXP2, a pore-forming protein required for the nutrient-permeable channel activity and protein export at the PVM. Unexpectedly, loss of EXP1 affected only the nutrient-permeable channel activity of the PVM but not protein export. Parasites with low levels of EXP1 became hypersensitive to low nutrient conditions, indicating that EXP1 indeed is needed for nutrient uptake and experimentally confirming the long-standing hypothesis that the channel activity measured at the PVM is required for parasite nutrient acquisition. Hence, EXP1 is specifically required for the functional expression of EXP2 as the nutrient-permeable channel and is critical for the metabolite supply of malaria parasites.
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