ArticleChembiochem : a European journal of chemical biology2020
A Method for High-Throughput Measurements of Viscosity in Sub-micrometer-Sized Membrane Systems.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Long-range viscosity of the plasma membrane of a living cell measured by a shear-driven flow method.Biophysical journal · 2025Article
- Examining the Impact of Storage Conditions on the Stability of a Liquid Formulation of mRNA-Loaded Lipid Nanoparticles.Pharmaceutics · 2025Article
- Temperature dependence of membrane viscosity of ternary lipid GUV with LBiophysical journal · 2025Article
- Phonons reveal coupled cholesterol-lipid dynamics in ternary membranes.Biophysical journal · 2024Article
- Gram-Positive Bacterial Membrane-Based Biosensor for Multimodal Investigation of Membrane-Antibiotic Interactions.Biosensors · 2024Article
- Protein Crowding and Cholesterol Increase Cell Membrane Viscosity in a Temperature Dependent Manner.Journal of chemical theory and computation · 2023Article
- Surface viscosities of lipid bilayers determined from equilibrium molecular dynamics simulations.Biophysical journal · 2023Article
- Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA.Small science · 2023Article
- A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes.Biophysical journal · 2022Article
- A Method for High-Throughput Measurements of Viscosity in Sub-micrometer-Sized Membrane Systems.Chembiochem : a European journal of chemical biology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To unravel the underlying principles of membrane adaptation in small systems like bacterial cells, robust approaches to characterize membrane fluidity are needed. Currently available relevant methods require advanced instrumentation and are not suitable for high-throughput settings needed to elucidate the biochemical pathways involved in adaptation. We developed a fast, robust, and financially accessible quantitative method to measure the microviscosity of lipid membranes in bulk suspension using a commercially available plate reader. Our approach, which is suitable for high-throughput screening, is based on the simultaneous measurements of absorbance and fluorescence emission of a viscosity-sensitive fluorescent dye, 9-(2,2-dicyanovinyl)julolidine (DCVJ), incorporated into a lipid membrane. We validated our method using artificial membranes with various lipid compositions over a range of temperatures and observed values that were in good agreement with previously published results. Using our approach, we were able to detect a lipid phase transition in the ruminant pathogen Mycoplasma mycoides.
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Registered trials
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