ArticleAnalytical methods : advancing methods and applications2019
Ultrasensitive Multi-Species Detection of CRISPR-Cas9 by a Portable Centrifugal Microfluidic Platform.
Article in Analytical methods : advancing methods and applications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Functional analysis of single enzymes combining programmable molecular circuits with droplet-based microfluidics.Nature nanotechnology · 2024Article
- Current Updates of CRISPR/Cas System and Anti-CRISPR Proteins: Innovative Applications to Improve the Genome Editing Strategies.International journal of nanomedicine · 2024Review
- The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure.Scientific reports · 2023Article
- Mechanisms regulating the CRISPR-Cas systems.Frontiers in microbiology · 2023Review
- A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1.Nature communications · 2022Article
- A miniaturized 3D printed pressure regulator (µPR) for microfluidic cell culture applications.Scientific reports · 2022Article
- Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.Nature reviews. Molecular cell biology · 2021Review
- CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip.Materials (Basel, Switzerland) · 2021Review
- Engineering fiber anisotropy within natural collagen hydrogels.American journal of physiology. Cell physiology · 2021Review
- Progress in the Application of Nanoparticles and Graphene as Drug Carriers and on the Diagnosis of Brain Infections.Molecules (Basel, Switzerland) · 2021Review
- Synergizing microfluidics with soft robotics: A perspective on miniaturization and future directions.Biomicrofluidics · 2021Article
- A 3D Printed Jet Mixer for Centrifugal Microfluidic Platforms.Micromachines · 2020Article
- Anti-CRISPRs: The natural inhibitors for CRISPR-Cas systems.Animal models and experimental medicine · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The discovery of the RNA-guided DNA nuclease CRISPR-Cas9 has enabled the targeted editing of genomes from diverse organisms, but the permanent and inheritable nature of genome modification also poses immense risks. The potential for accidental exposure, malicious use, or undesirable persistence of Cas9 therapeutics and off-target genome effects highlight the need for detection assays. Here we report a centrifugal microfluidic platform for the measurement of both Cas9 protein levels and nuclease activity. Because Cas9 from many bacterial species have been adapted for biotechnology applications, we developed the capability to detect Cas9 from the widely-used
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.