ArticleDevelopmental cell2019
Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms.
Article in Developmental cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Cellular Morphometric Analysis (CellMorph)-a comprehensive imaging-based tool for quantifying cellular phenotype heterogeneity and dynamics across biological processes.The FEBS journal · 2026Article
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- Comprehensive Analysis of Drug Response using the FLICK Assay.Journal of visualized experiments : JoVE · 2025Article
- Molecular glues of the regulatory ChREBP/14-3-3 complex protect beta cells from glucolipotoxicity.Nature communications · 2025Article
- Metabolic adaptations to acute glucose uptake inhibition converge upon mitochondrial respiration for leukemia cell survival.Cell communication and signaling : CCS · 2025Article
- Molecular glues of the regulatory ChREBP/14-3-3 complex protect beta cells from glucolipotoxicity.bioRxiv : the preprint server for biology · 2024Article
- Dynamic death decisions: How mitochondrial dynamics shape cellular commitment to apoptosis and ferroptosis.Developmental cell · 2024Review
- Quick tips for interpreting cell death experiments.Nature cell biology · 2023Article
- Apoptotic volume decrease (AVD) in AFrontiers in physiology · 2023Article
- IFN-γCellular & molecular immunology · 2022Article
- T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.JCI insight · 2021Article
- Preclinical Drug Response Metric Based on Cellular Response Phenotype Provides Better Pharmacogenomic Variables with Phenotype Relevance.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Immune dysregulation in SHARPIN-deficient mice is dependent on CYLD-mediated cell death.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL).STAR protocols · 2020Article
- Cell death through the ages: The ICDS 25th Anniversary Meeting.The FEBS journal · 2020Article
- Why not add some SPARKL to your life (and death)!?Molecular & cellular oncology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Quantifying cytostatic and cytotoxic outcomes are integral components of characterizing perturbagens used as research tools and in drug discovery pipelines. Furthermore, data-rich acquisition, coupled with robust methods for analysis, is required to properly assess the function and impact of these perturbagens. Here, we present a detailed and versatile method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). SPARKL integrates high-content live-cell imaging with automated detection and analysis of fluorescent reporters of cell death. We outline several examples of zero-handling, non-disruptive protocols for detailing cell death mechanisms and proliferation profiles. Additionally, we suggest several methods for mathematically analyzing these data to best utilize the collected kinetic data. Compared to traditional methods of detection and analysis, SPARKL is more sensitive, accurate, and high throughput while substantially eliminating sample processing and providing richer data.
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Registered trials
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