ArticleBMC research notes2019
microRNA-92a regulates the expression of aphid bacteriocyte-specific secreted protein 1.
Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- mir-31 mediated control of bacteriome size in tsetse flies.Current research in insect science · 2025Article
- Comparative transcriptomics of aphid species that diverged > 22 MYA reveals genes that are important for the maintenance of their symbiosis.Scientific reports · 2023Article
- Symbiosis preservation: Putative regulation of fatty acyl-CoA reductase by miR-31a within the symbiont harboring bacteriome through tsetse evolution.Frontiers in microbiology · 2023Article
- The Tsetse Metabolic Gambit: Living on Blood by Relying on Symbionts Demands Synchronization.Frontiers in microbiology · 2022Review
- Article
- JNK pathway plays a key role in the immune system of the pea aphid and is regulated by microRNA-184.PLoS pathogens · 2020Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
objectiveAphids harbor a nutritional obligate endosymbiont in specialized cells called bacteriocytes, which aggregate to form an organ known as the bacteriome. Aphid bacteriomes display distinct gene expression profiles that facilitate the symbiotic relationship. Currently, the mechanisms that regulate these patterns of gene expression are unknown. Recently using computational pipelines, we identified miRNAs that are conserved in expression in the bacteriomes of two aphid species and proposed that they function as important regulators of bacteriocyte gene expression. Here using a dual luciferase assay in mouse NIH/3T3 cell culture, we aimed to experimentally validate the computationally predicted interaction between Myzus persicae miR-92a and the predicted target region of M. persicae bacteriocyte-specific secreted protein 1 (SP1) mRNA.
resultsIn the dual luciferase assay, miR-92a interacted with the SP1 target region resulting in a significant downregulation of the luciferase signal. Our results demonstrate that miR-92a interacts with SP1 to alter expression in a heterologous expression system, thereby supporting our earlier assertion that miRNAs are regulators of the aphid/Buchnera symbiotic interaction.
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Registered trials
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