ArticleJournal of experimental botany2019
CRISPR/Cas9-mediated targeted mutagenesis for functional genomics research of crassulacean acid metabolism plants.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Rhythmic Mechanisms Governing CAM Photosynthesis inInternational journal of molecular sciences · 2026Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Transformative Potential of Induced Pluripotent Stem Cells in Congenital Heart Disease Research and Treatment.Children (Basel, Switzerland) · 2025Review
- A split ribozyme system for in vivo plant RNA imaging and genetic engineering.Plant biotechnology journal · 2025Article
- Bringing CAM photosynthesis to the table: Paving the way for resilient and productive agricultural systems in a changing climate.Plant communications · 2024Review
- Article
- Prospects and perspectives: inferring physiological and regulatory targets for CAM from molecular and modelling approaches.Annals of botany · 2023Review
- Diel dynamics of multi-omics in elkhorn fern provide new insights into weak CAM photosynthesis.Plant communications · 2023Article
- Defining Mechanisms of CInternational journal of molecular sciences · 2023Review
- Article
- Advances and perspectives in discovery and functional analysis of small secreted proteins in plants.Horticulture research · 2021Review
- Review
- Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research.Biodesign research · 2020Review
- Editorial: Systems Biology and Synthetic Biology in Relation to Drought Tolerance or Avoidance in Plants.Frontiers in plant science · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Biosystems Design to Accelerate CBiodesign research · 2020Review
- Comparative genomics can provide new insights into the evolutionary mechanisms and gene function in CAM plants.Journal of experimental botany · 2019Review
- CRISPR/Cas9-mediated targeted mutagenesis for functional genomics research of crassulacean acid metabolism plants.Journal of experimental botany · 2019Article
- New perspectives on crassulacean acid metabolism biology.Journal of experimental botany · 2019Review
- Rol-Transformed Kalanchoë Shows Altered Root Allocation and Photosynthetic Responses to Nitrogen Deficiency.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crassulacean acid metabolism (CAM) is an important photosynthetic pathway in diverse lineages of plants featuring high water-use efficiency and drought tolerance. A big challenge facing the CAM research community is to understand the function of the annotated genes in CAM plant genomes. Recently, a new genome editing technology using CRISPR/Cas9 has become a more precise and powerful tool than traditional approaches for functional genomics research in C3 and C4 plants. In this study, we explore the potential of CRISPR/Cas9 to characterize the function of CAM-related genes in the model CAM species Kalanchoë fedtschenkoi. We demonstrate that CRISPR/Cas9 is effective in creating biallelic indel mutagenesis to reveal previously unknown roles of blue light receptor phototropin 2 (KfePHOT2) in the CAM pathway. Knocking out KfePHOT2 reduced stomatal conductance and CO2 fixation in late afternoon and increased stomatal conductance and CO2 fixation during the night, indicating that blue light signaling plays an important role in the CAM pathway. Lastly, we provide a genome-wide guide RNA database targeting 45 183 protein-coding transcripts annotated in the K. fedtschenkoi genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.