ArticleNature methods2019
Laser phase plate for transmission electron microscopy.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 198 citations in OpenAlex.
- EXPECTATION-MAXIMIZATION FOR STRUCTURE DETERMINATION DIRECTLY FROM CRYO-EM MICROGRAPHS.Inverse problems and imaging (Springfield, Mo.) · 2027Article
- High-resolution nuclear cell biology by cryo-electron tomography.Nucleus (Austin, Tex.) · 2026Review
- A practical guide to investigating biomolecular condensates: a comment from the plant community.Science China. Life sciences · 2026Review
- When a laser beam hits an electron beam.Nature methods · 2026Article
- Elastic quantum coupling between free electrons and photons.Science advances · 2026Article
- Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae.bioRxiv : the preprint server for biology · 2026Article
- Laser phase plate improves structure determination of small proteins by cryo-EM.Science (New York, N.Y.) · 2026Article
- Article
- Crossed laser phase plates for transmission electron microscopy.Nature communications · 2026Article
- Heterogeneous reconstruction algorithms for cryoEM achieve limited particle classification accuracy on real benchmark datasets.bioRxiv : the preprint server for biology · 2026Article
- Electrons and X-rays for diffraction and imaging.Acta crystallographica. Section D, Structural biology · 2026Review
- Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography.IUCrJ · 2026Article
- Damage-limited resolution for X-ray and electron microscopy of organic specimens.Acta crystallographica. Section D, Structural biology · 2026Article
- Biological Breakthroughs and Drug Discovery Revolution via Cryo-Electron Microscopy of Membrane Proteins.Membranes · 2025Review
- Realization of a Pre-Sample Photonic-Based Free-Electron Modulator in Ultrafast Transmission Electron Microscopes.ACS photonics · 2025Article
- Chromatic aberration (Cc) corrected cryo-EM: The structure of pseudorabies virus (PRV) using both zero-loss and energy loss electrons.Ultramicroscopy · 2025Article
- Roadmap for Quantum Nanophotonics with Free Electrons.ACS photonics · 2025Review
- Ultrafast 4D Scanning Transmission Electron Microscopy for Imaging of Localized Optical Fields.ACS photonics · 2025Article
- Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography.Structural dynamics (Melville, N.Y.) · 2025Article
- Illuminating ubiquitination mechanisms: How cryo-EM has shed light on Cullin RING E3 ligase function.Current opinion in structural biology · 2025Review
23 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Here, we demonstrate electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. We demonstrate the laser phase plate by imaging an amorphous carbon film. The laser phase plate provides a stable and tunable phase shift without electrostatic charging or unwanted electron scattering. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.