ArticleScientific reports2019
Identification of reference genes for RT-qPCR data normalisation in aging studies.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 48 citations in OpenAlex.
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- Age-associated increases in inter-individual gene expression variability across human tissues.bioRxiv : the preprint server for biology · 2026Article
- A bioinformatic survey of RNA isoform diversity and expression across 9 GTEx tissues using long-read sequencing data.BMC genomics · 2025Article
- Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic AlkalithermophileMicroorganisms · 2025Article
- Senescence-Associated Chromatin Rewiring Promotes Inflammation and Transposable Element Activation.bioRxiv : the preprint server for biology · 2025Article
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- Age-invariant genes: multi-tissue identification and characterization of murine reference genes.Aging · 2025Article
- Identification of new reference genes with stable expression patterns for cell cycle experiments in human leukemia cell lines.Scientific reports · 2025Article
- Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.Epigenomics · 2025Article
- Evaluation of candidate RT-qPCR reference genes in the aging African turquoise killifish brain.Aging brain · 2025Article
- A comparative assessment of reference genes in mouse brown adipocyte differentiation and thermogenesis in vitro.Adipocyte · 2024Article
- Gene expression & biochemical analysis in alkaptonuria caused by a founder pathogenic variant across different age groups from India.The Indian journal of medical research · 2024Article
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- The N-acetylglucosaminyltransferase Radical fringe contributes to defects in JAG1-dependent turnover and signaling of NOTCH3 CADASIL mutants.The Journal of biological chemistry · 2024Article
- Identification of a circulating long non-coding RNA signature panel in plasma as a novel biomarker for the detection of acute/early-stage HIV-1 infection.Biomarker research · 2024Article
- Age-Invariant Genes: Multi-Tissue Identification and Characterization of Murine Reference Genes.bioRxiv : the preprint server for biology · 2024Article
- Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions inPlants (Basel, Switzerland) · 2024Article
- Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR.Scientific reports · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aging is associated with changes in gene expression levels that affect cellular functions and predispose to age-related diseases. The use of candidate genes whose expression remains stable during aging is required to correctly address the age-associated variations in expression levels. Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) has become a powerful approach for sensitive gene expression analysis. Reliable RT-qPCR assays rely on the normalisation of the results to stable reference genes. Taken these data together, here we evaluated the expression stability of eight frequently used reference genes in three aging models: oncogene-induced senescence (OIS), in vitro and in vivo aging. Using NormFinder and geNorm algorithms, we identified that the most stable reference gene pairs were PUM1 and TBP in OIS, GUSB and PUM1 for in vitro aging and GUSB and OAZ1 for in vivo aging. To validate these candidates, we used them to normalise the expression data of CDKN1A, APOD and TFRC genes, whose expression is known to be affected during OIS, in vitro and in vivo aging. This study demonstrates that accurate normalisation of RT-qPCR data is crucial in aging research and provides a specific subset of stable reference genes for future aging studies.
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