Evidence map›Paper›PMID 31562216›Full record

ArticleThe Plant cell2019

CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis.

Ward Decaestecker, Rafael Andrade Buono, Marie L Pfeiffer, Nick Vangheluwe, Joris Jourquin, Mansour Karimi, Gert Van Isterdael, Tom Beeckman, Moritz K Nowack, Thomas B Jacobs

Open access · bronzeAbstract read
In one paragraph

Article in The Plant cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.

0numbers the graph read from it
0cells of the map it votes in
128citing papers in PubMed
12.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

128 citing papers in PubMed, 253 citations in OpenAlex.

  1. Gene Expression Analysis from Specific Root Cell Types.Methods in molecular biology (Clifton, N.J.) · 2027
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68 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Ward DecaesteckerDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-0247-3189
Rafael Andrade BuonoDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-6675-3836
Marie L PfeifferDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-5360-054X
Nick VangheluweDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-4885-6161
Joris JourquinDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-0808-0730
Mansour KarimiDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-0246-9318
Gert Van IsterdaelVIB Flow Core, VIB Center for Inflammation Research, Technologiepark 71, B-9052 Ghent, Belgium.ORCID 0000-0001-6626-1316
Tom BeeckmanDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0001-8656-2060
Moritz K NowackDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium thomas.jacobs@vib.be moritz.nowack@vib.be.ORCID 0000-0001-8918-7577
Thomas B JacobsDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium thomas.jacobs@vib.be moritz.nowack@vib.be.ORCID 0000-0002-5408-492X
Ghent University · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Detailed functional analyses of many fundamentally important plant genes via conventional loss-of-function approaches are impeded by the severe pleiotropic phenotypes resulting from these losses. In particular, mutations in genes that are required for basic cellular functions and/or reproduction often interfere with the generation of homozygous mutant plants, precluding further functional studies. To overcome this limitation, we devised a clustered regularly interspaced short palindromic repeats (CRISPR)-based tissue-specific knockout system, CRISPR-TSKO, enabling the generation of somatic mutations in particular plant cell types, tissues, and organs. In Arabidopsis (

Indexed as

MutagenesisArabidopsisCloning, MolecularClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsCyclin-Dependent KinasesGene Knockout TechniquesGenetic VectorsOrgan SpecificityPhenotypePlant Root CapPlant RootsPlant StomataPromoter Regions, GeneticCyclin-Dependent Kinases

Identifiers

PMID31562216
PMCPMC6925012
OpenAlexW2975935187

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.