ArticleInternational journal of molecular sciences2019
Optimal Isolation Method of Small Extracellular Vesicles from Rat Plasma.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Engineering the Future: Strategic Advances in Extracellular Vesicle-Mediated Drug Delivery Systems.International journal of nanomedicine · 2026Review
- Comparative study on drug encapsulation and release kinetics in extracellular vesicles loaded with snake venom L - amino acid oxidase.BMC pharmacology & toxicology · 2025Article
- Development of an Effective Isolation Method for Plasma Extracellular Vesicles Excluding Lipoproteins by Precipitation With Polyethylene Glycol.Journal of extracellular biology · 2025Article
- Autocrine role of senescent cardiac fibroblasts-derived extracellular vesicles.The Journal of veterinary medical science · 2023Article
- Frontier Review of the Molecular Mechanisms and Current Approaches of Stem Cell-Derived Exosomes.Cells · 2023Review
- Exploring Purification Methods of Exosomes from Different Biological Samples.BioMed research international · 2023Article
- Comparative analysis of extracellular vesicle isolation methods from human AML bone marrow cells and AML cell lines.Frontiers in oncology · 2022Article
- Serum extracellular vesicle miR-203a-3p content is associated with skeletal muscle mass and protein turnover during disuse atrophy and regrowth.American journal of physiology. Cell physiology · 2020Article
- Plasma small extracellular vesicles in hypertensive rats impair reactivity of isolated blood vessels.The Journal of veterinary medical science · 2020Article
- Small extracellular vesicles from rat plasma promote migration and proliferation of vascular smooth muscle cells.The Journal of veterinary medical science · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small extracellular vesicles (sEVs) mediate cell-to-cell communication. We recently reported that circulating sEVs regulate systolic blood pressure in an animal model of human systemic hypertension. However, the underlying mechanisms still remain to be elucidated. As the first step for detailed analyses, we sought to increase the yield and purity of sEVs isolated from rat plasma. We compared the concentration and size distribution of sEVs as well as protein expression of the sEV marker and contaminants among plasma sEVs isolated by the ultracentrifugation (UC) method, the precipitation with polyethylene-glycol and ultracentrifugation (PEG-UC) method, or the precipitation with polyethylene-glycol (PEG) method. Effects of anticoagulants were also examined. The total concentration of plasma sEVs isolated by the PEG or PEG-UC method was much higher than that of the UC method. In the plasma sEVs isolated by the PEG-UC method, contaminating proteins were lower, while the protein expression of certain sEV markers was higher than that of the PEG method. There was no significant difference in total concentration or protein expression of sEV markers in sEVs isolated from rat plasma treated with three different anticoagulants (heparin, ethylenediaminetetraacetic acid, or acid citrate dextrose buffer) by the PEG-UC method. We, for the first time, determined that the PEG-UC method was optimal for sEV isolation from rat plasma.
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