ArticleFungal biology and biotechnology2019
Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi.
Article in Fungal biology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 113 citations in OpenAlex.
- Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families inCell surface (Amsterdam, Netherlands) · 2025Article
- Time-resolved analysis of Bacillus subtilis DB104 Spo0A-mutant transcriptome profile and enhancement of recombinant protein release.Journal of microbiology (Seoul, Korea) · 2025Article
- Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2025Review
- Competition between homologous chromosomal DNA and exogenous donor DNA to repair CRISPR/Cas9-induced double-strand breaks in Aspergillus niger.Fungal biology and biotechnology · 2024Article
- Increasing the efficiency of CRISPR/Cas9-mediated genome editing in the citrus postharvest pathogen Penicillium digitatum.Fungal biology and biotechnology · 2024Article
- The role of the Flb protein family in the life cycle of Aspergillus niger.Antonie van Leeuwenhoek · 2024Article
- Expanding the Toolbox for Genetic Manipulation inJournal of fungi (Basel, Switzerland) · 2024Article
- CRISPR/Cas9 system is a suitable gene targeting editing tool to filamentous fungus Monascus pilosus.Applied microbiology and biotechnology · 2024Article
- Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2.Biotechnology letters · 2023Article
- Compatible solutes determine the heat resistance of conidia.Fungal biology and biotechnology · 2023Article
- CRISPR/Cas9-Mediated Disruption of theJournal of fungi (Basel, Switzerland) · 2023Article
- The α-(1,3)-glucan synthase gene agsE impacts the secretome of Aspergillus niger.Antonie van Leeuwenhoek · 2023Article
- A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus SectionMicrobiology spectrum · 2023Article
- Identification of Compounds PreventingInternational journal of molecular sciences · 2023Article
- Article
- How to Completely Squeeze a Fungus-Advanced Genome Mining Tools for Novel Bioactive Substances.Pharmaceutics · 2022Review
- Genome sequences of 24 Aspergillus niger sensu stricto strains to study strain diversity, heterokaryon compatibility, and sexual reproduction.G3 (Bethesda, Md.) · 2022Article
- Efficient genome editing inSynthetic and systems biotechnology · 2022Article
- High sorbic acid resistance of Penicillium roqueforti is mediated by the SORBUS gene cluster.PLoS genetics · 2022Article
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are time-consuming and can be of mutagenic nature. These developments allow the study of large gene families that contain putatively redundant genes, such as the seven-membered family of
resultsHere, we present a CRISPR/Cas9 system for
conclusionsOur findings advocate the use of CRISPR/Cas9 to create multiple gene deletions in both a fast and reliable way, while simultaneously omitting possible locus-dependent-side-effects of poor auxotrophic marker expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.