ArticleNature communications2019
Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 91 citations in OpenAlex.
- Advancement of Peptide-PAINT: Applications and Current Challenges in Super-Resolution Imaging.Small science · 2026Review
- Fluorescence localization and tracking imaging with a spectral-splitting perovskite single-pixel detector.Nature communications · 2026Article
- Optically switchable CLEAR probes enable rapid, biocompatible and high-efficiency fluorophore exchange for ultra-plex, high-resolution immunofluorescence imaging.Chemical science · 2026Article
- Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.ACS nano · 2026Article
- Utilizing the predictable binding kinetics of DNA-PAINT to denoise super-resolution images.Nature communications · 2026Article
- Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body.Nature communications · 2025Article
- Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.Nature communications · 2025Article
- Article
- Understanding the molecular diversity of synapses.Nature reviews. Neuroscience · 2025Review
- Data-driven synapse classification reveals a logic of glutamate receptor diversity.bioRxiv : the preprint server for biology · 2025Article
- Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions.Nucleic acids research · 2024Article
- Spatial Multi-Omics in Alzheimer's Disease: A Multi-Dimensional Approach to Understanding Pathology and Progression.Current issues in molecular biology · 2024Review
- Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states.Nature communications · 2024Article
- Multidimensional analysis of cortical interneuron synaptic features reveals underlying synaptic heterogeneity.bioRxiv : the preprint server for biology · 2024Article
- Metabolic Bypass Rescues Aberrant S-nitrosylation-Induced TCA Cycle Inhibition and Synapse Loss in Alzheimer's Disease Human Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy.Cell reports methods · 2023Article
- Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage.ACS bio & med chem Au · 2023Article
- A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.Cell reports · 2023Article
- Automated high-content imaging in iPSC-derived neuronal progenitors.SLAS discovery : advancing life sciences R & D · 2023Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
Synapses contain hundreds of distinct proteins whose heterogeneous expression levels are determinants of synaptic plasticity and signal transmission relevant to a range of diseases. Here, we use diffusible nucleic acid imaging probes to profile neuronal synapses using multiplexed confocal and super-resolution microscopy. Confocal imaging is performed using high-affinity locked nucleic acid imaging probes that stably yet reversibly bind to oligonucleotides conjugated to antibodies and peptides. Super-resolution PAINT imaging of the same targets is performed using low-affinity DNA imaging probes to resolve nanometer-scale synaptic protein organization across nine distinct protein targets. Our approach enables the quantitative analysis of thousands of synapses in neuronal culture to identify putative synaptic sub-types and co-localization patterns from one dozen proteins. Application to characterize synaptic reorganization following neuronal activity blockade reveals coordinated upregulation of the post-synaptic proteins PSD-95, SHANK3 and Homer-1b/c, as well as increased correlation between synaptic markers in the active and synaptic vesicle zones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.