Evidence map›Paper›PMID 31558745›Full record

ArticleScientific reports2019

How to copy and paste DNA microarrays.

Stefan D Krämer, Johannes Wöhrle, Philipp A Meyer, Gerald A Urban, Günter Roth

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Stefan D KrämerZBSA - Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg, Habsburgerstrasse. 49, D-79104, Freiburg, Germany. stefan.kraemer@biocopy.de.ORCID https://orcid.org/0000-0002-0071-9344
Johannes WöhrleZBSA - Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg, Habsburgerstrasse. 49, D-79104, Freiburg, Germany.ORCID https://orcid.org/0000-0001-5350-5596
Philipp A MeyerZBSA - Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg, Habsburgerstrasse. 49, D-79104, Freiburg, Germany.ORCID https://orcid.org/0000-0002-9926-5732
Gerald A UrbanIMTEK - Dep. of Microsystems Engineering, Albert-Ludwigs-University Freiburg, Georges-Köhler-Allee 103, D-79110, Freiburg, Germany.ORCID https://orcid.org/0000-0003-1567-000X
Günter RothZBSA - Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg, Habsburgerstrasse. 49, D-79104, Freiburg, Germany.ORCID https://orcid.org/0000-0001-9343-6046

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.

Indexed as

Costs and Cost AnalysisDNA ProbesDNA, Single-StrandedHumansOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionThrombinDNA ProbesDNA, Single-StrandedThrombin

Identifiers

PMID31558745
PMCPMC6763488

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.