ArticleAllergy2020
Sputum microbiomic clustering in asthma and chronic obstructive pulmonary disease reveals a Haemophilus-predominant subgroup.
Article in Allergy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- The Human Respiratory Microbiome: Current Understandings and Future Directions.American journal of respiratory cell and molecular biology · 2023Pooled it
- Effects of Inhaled Corticosteroids/Long-Acting Beta-Agonists (ICS/LABA) on Airway Microbial Diversity and IL-8/IL-10 Cytokine Levels in Stable COPD.Combinatorial chemistry & high throughput screening · 2026Article
- Interactions between upper respiratory microbiota and psychological distress in pulmonary nodule patients: a retrospective nested case-control study based on machine learning and exploratory mediation analysis.International journal of surgery (London, England) · 2025Article
- Sputum quality affects assessment of airway microbiology in childhood asthma.Respiratory research · 2025Article
- Sputum sample quality selection in microbiota research; a chance to improve methods??Respiratory research · 2025Article
- The impact of environmental factors on respiratory tract microbiome and respiratory system diseases.European journal of medical research · 2025Review
- The Causal Relationship Between Gut and Skin Microbiota and Chronic Obstructive Pulmonary Disease:A Bidirectional Two-Sample Mendelian Randomization Analysis.International journal of chronic obstructive pulmonary disease · 2025Article
- Species-level, metagenomic and proteomic analysis of microbe-immune interactions in severe asthma.Allergy · 2024Article
- A severe asthma phenotype of excessive airway Haemophilus influenzae relative abundance associated with sputum neutrophilia.Clinical and translational medicine · 2024Article
- Respiratory Microbiome Profiles Associated With Distinct Inflammatory Phenotype and Clinical Indexes in Chronic Obstructive Pulmonary Disease.Chronic obstructive pulmonary diseases (Miami, Fla.) · 2024Article
- Sputum microbiota and inflammatory subtypes in asthma, COPD, and its overlap.The journal of allergy and clinical immunology. Global · 2024Article
- Article
- Clinical Trial Design Innovations for Precision Medicine in Asthma.Advances in experimental medicine and biology · 2023Article
- MAIT cells and the microbiome.Frontiers in immunology · 2023Review
- Review
- Sputum induction and its diagnostic applications in inflammatory airway disorders: a review.Frontiers in allergy · 2023Review
- Non-typeableEuropean respiratory review : an official journal of the European Respiratory Society · 2022Review
- Validation of Sputum Biomarker Immunoassays and Cytokine Expression Profiles in COPD.Biomedicines · 2022Article
- Effect of inhaled corticosteroids on microbiome and microbial correlations in asthma over a 9-month period.Clinical and translational science · 2022Article
- Topological data analysis in biomedicine: A review.Journal of biomedical informatics · 2022Review
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 2 countries.
Funding
Abstract
backgroundAirway ecology is altered in asthma and chronic obstructive pulmonary disease (COPD). Anti-microbial interventions might have benefit in subgroups of airway disease. Differences in sputum microbial profiles at acute exacerbation of airways disease are reflected by the γProteobacteria:Firmicutes (γP:F) ratio. We hypothesized that sputum microbiomic clusters exist in stable airways disease, which can be differentiated by the sputum γP:F ratio.
methodsSputum samples were collected from 63 subjects with severe asthma and 78 subjects with moderate-to-severe COPD in a prospective single centre trial. Microbial profiles were obtained through 16S rRNA gene sequencing. Topological data analysis was used to visualize the data set and cluster analysis performed at genus level. Clinical characteristics and sputum inflammatory mediators were compared across the clusters.
resultsTwo ecological clusters were identified across the combined airways disease population. The smaller cluster was predominantly COPD and was characterized by dominance of Haemophilus at genus level (n = 20), high γP:F ratio, increased H influenzae, low diversity measures and increased pro-inflammatory mediators when compared to the larger Haemophilus-low cluster (n = 121), in which Streptococcus demonstrated the highest relative abundance at the genus level. Similar clusters were identified within disease groups individually and the γP:F ratio consistently differentiated between clusters.
conclusionCluster analysis by airway ecology of asthma and COPD in stable state identified two subgroups differentiated according to dominance of Haemophilus. The γP:F ratio was able to distinguish the Haemophilus-high versus Haemophilus-low subgroups, whether the Haemophilus-high group might benefit from treatment strategies to modulate the airway ecology warrants further investigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.