ReviewFrontiers in plant science2019
DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification.
Review in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Species Determination Within the Felidae Family Using mtDNA Minibarcoding.Animal genetics · 2026Article
- DNA barcoding and phylogenetic insights into the selected endemic flora of the Western Himalayas.Scientific reports · 2026Article
- Advancing plant DNA barcoding: integrating chloroplast genome sequencing, cryptic diversity discovery and machine learning.Molecular biology reports · 2026Review
- Establishing a TaqMan qPCR method for identification and quantification ofFrontiers in pharmacology · 2026Article
- Plastome diversity and phylogenetic insights among modern Egyptian wheat cultivars: Genome-Wide and Gene-Level perspectives.BMC plant biology · 2025Article
- Analytical assessment of physical characteristics, metabolic processes, and molecular investigations of selected wheat (Triticum spp.) cultivars.BMC plant biology · 2025Article
- A composite universal DNA signature for the tree of life.Nature ecology & evolution · 2025Article
- Development of a mitochondrial mini-barcode and its application in metabarcoding for identification of leech in traditional Chinese medicine.Scientific reports · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Heliyon · 2024Article
- Molecular identification and studies on genetic diversity and structure-related GC heterogeneity of Spatholobus Suberectus based on ITS2.Scientific reports · 2024Article
- Flower Species Ingredient Verification Using Orthogonal Molecular Methods.Foods (Basel, Switzerland) · 2024Article
- Molecular authentication of commercial "Qian-hu" through the integration of nrDNA internal transcribed spacer 2 and nucleotide signature.Molecular biology reports · 2024Article
- Article
- DNA Sequencing Technologies and DNA Barcoding.Methods in molecular biology (Clifton, N.J.) · 2024Article
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM.BMC plant biology · 2023Article
- Genetic characterization of a group of commercial African timber species: From genomics to barcoding.PloS one · 2023Article
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
- A specific SNP-based multiplex PCR assay for the simultaneous identification of two biological ingredients for the Chinese patent medicine, Danggui Buxue pill.Frontiers in pharmacology · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, the demand for natural herbal products (NHP) has increased; however, the quality of these products is difficult to confirm due to the lack of a comprehensive quality control system. Traditional methods are not effective in detecting processed ingredients. DNA barcoding is an established technique that has been used for more than 10 years. This technique uses short standard sequences (generally 200-600 bp) to identify species. While a complete DNA barcode is difficult to obtain from NHP due to DNA degradation, mini-barcoding is a complementary tool to identify species in NHP. DNA mini-barcoding uses smaller DNA segments for polymerase chain reaction amplification and can be applied to identify species rapidly. The present review summarizes the development and application of DNA mini-barcodes over recent years and discusses the limitations of this technique. This review also compares mini-barcoding and meta-barcoding, a technique using universal polymerase chain reaction primers to simultaneously amplify multiple DNA barcodes and identify many species in a single environmental sample. Additionally, other detection methods that can be combined with mini-barcodes, such as nucleotide signatures, high-resolution DNA melting analysis, and gold nanoparticles, are discussed. DNA mini-barcoding can fill the gaps left by other methods in the field of herbal molecular identification.
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