Evidence map›Paper›PMID 31555273›Full record

ArticleFrontiers in immunology2019

Short-Chain Fatty Acids Regulate the Immune Responses via G Protein-Coupled Receptor 41 in Bovine Rumen Epithelial Cells.

Kang Zhan, Xiaoxiao Gong, Yinyin Chen, Maocheng Jiang, Tianyu Yang, Guoqi Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 54 citations in OpenAlex.

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  16. GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kang ZhanInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Xiaoxiao GongInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Yinyin ChenInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Maocheng JiangInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Tianyu YangInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Guoqi ZhaoInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Yangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rumen immune system often suffers when challenging antigens from lysis of dead microbiota cells in the rumen. However, the rumen epithelium innate immune system can actively respond to the infection. Previous studies have demonstrated G protein-coupled receptors 41 (GPR41) as receptors for short chain fatty acids (SCFAs) in human. We hypothesized that SCFAs, the most abundant microbial metabolites in rumen, may regulate the immune responses by GPR41 in bovine rumen epithelial cells (BRECs). Therefore, the objective of study was to firstly establish an immortal BRECs line and investigate the regulatory effects of SCFAs and GPR41 on innate immunity responses in BRECs. These results showed that long-term BRECs cultures were established by SV40T-induced immortalization. The concentrations of 20 mM SCFAs significantly enhanced the levels of GPR41, IL1β, TNFα, chemokines, and immune barrier genes by transcriptome analysis. Consistent with transcriptome results, the expression of GPR41, IL1β, TNFα, and chemokines were markedly upregulated in BRECs treated with 20 mM SCFAs by qRT-PCR compared with control BRECs. Remarkably, the GPR41 knockdown (GPR41KD) BRECs treated with 20 mM SCFAs significantly enhanced the proinflammatory cytokines IL1β and TNFα expression compared with wild type BRECs treated with 20 mM SCFAs, but reduced the expression of CCL20, CXCL2, CXCL3, CXCL5, CXCL8, CXCL14, Occludin, and ZO-1. Moreover, GPR41 mRNA expression is positively correlated with CCL20, CXCL2, CXCL3, CXCL8, CXCL14, and ZO-1. These findings revealed that SCFAs regulate GPR41-mediated levels of genes involved in immune cell recruitment and epithelial immune barrier and thereby mediate protective innate immunity in BRECs.

Indexed as

AnimalsCattleChemokinesEpithelial CellsFatty Acids, VolatileGastric MucosaGene Expression ProfilingGene Knockout TechniquesHigh-Throughput Nucleotide SequencingInflammation MediatorsReceptors, G-Protein-CoupledRumenTight Junction ProteinsChemokinesFatty Acids, VolatileInflammation MediatorsReceptors, G-Protein-CoupledTight Junction Proteinsbovine rumen epithelial cellsGPR41immortalizationimmune responsesshort-chain fatty acids

Identifiers

PMID31555273
PMCPMC6722193
OpenAlexW2968979230

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.