ArticleCurrent genomics2019
OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells.
Article in Current genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Genome-wide phylogenetic analysis and expression profiling of F-box gene family in Verbena bonariensis under cold stress.BMC plant biology · 2026Article
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- Identification of miRNAs in Response to Cold Stress in 'Chaling' Common Wild Rice (Life (Basel, Switzerland) · 2025Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Rice-specific miR1850.1 targets NPR3 to regulate cold stress response.Plant communications · 2025Article
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Plants (Basel, Switzerland) · 2024Article
- MicroRNA2871b of Dongxiang Wild Rice (International journal of molecular sciences · 2023Article
- Article
- Navigating rice seedling cold resilience: QTL mapping in two inbred line populations and the search for genes.Frontiers in plant science · 2023Article
- Research progress on plant noncoding RNAs in response to low-temperature stress.Plant signaling & behavior · 2022Review
- Osa-miR1320 targets the ERF transcription factor OsERF096 to regulate cold tolerance via JA-mediated signaling.Plant physiology · 2022Article
- MicroRNA-mediated host defense mechanisms against pathogens and herbivores in rice: balancing gains from genetic resistance with trade-offs to productivity potential.BMC plant biology · 2022Review
- The key micronutrient copper orchestrates broad-spectrum virus resistance in rice.Science advances · 2022Article
- The miR528-D3 Module Regulates Plant Height in Rice by Modulating the Gibberellin and Abscisic Acid Metabolisms.Rice (New York, N.Y.) · 2022Article
- Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology.Frontiers in nutrition · 2022Article
- Small RNA Sequencing Revealed that miR4415, a Legume-Specific miRNA, was Involved in the Cold Acclimation ofFrontiers in genetics · 2022Article
- Intragenic heterochromatin-mediated alternative polyadenylation modulates miRNA and pollen development in rice.The New phytologist · 2021Article
- Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat.International journal of molecular sciences · 2020Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs participate in many molecular mechanisms and signaling trans-duction pathways that are associated with plant stress tolerance by repressing expression of their target genes. However, how microRNAs enhance tolerance to low temperature stress in plant cells remains elusive.
objectiveIn this investigation, we demonstrated that overexpression of the rice microRNA528 (Os-miR528) increases cell viability, growth rate, antioxidants content, ascorbate peroxidase (APOX) activi-ty, and superoxide dismutase (SOD) activity and decreases ion leakage rate and thiobarbituric acid reac-tive substances (TBARS) under low temperature stress in Arabidopsis (Arabidopsis thaliana), pine (Pi-nus elliottii), and rice (Oryza sativa).
methodsTo investigate the potential mechanism of OsmiR528 in increasing cold stress tolerance, we examined expression of stress-associated MYB transcription factors OsGAMYB-like1, OsMYBS3, OsMYB4, OsMYB3R-2, OsMYB5, OsMYB59, OsMYB30, OsMYB1R, and OsMYB20 in rice cells by qRT-PCR.
resultsOur experiments demonstrated that OsmiR528 decreases expression of transcription factor OsMYB30 by targeting a F-box domain containing protein gene (Os06g06050), which is a positive regulator of OsMYB30. In OsmiR528 transgenic rice, reduced OsMYB30 expression results in in-creased expression of BMY genes OsBMY2, OsBMY6, and OsBMY10. The transcript levels of the OsBMY2, OsBMY6, and OsBMY10 were elevated by OsMYB30 knockdown, but decreased by Os-MYB30 overexpression in OsmiR528 transgenic cell lines, suggesting that OsmiR528 increases low temperature tolerance by modulating expression of stress response-related transcription factor.
conclusionOur experiments provide novel information in increasing our understanding in molecular mechanisms of microRNAs-associated low temperature tolerance and are valuable in plant molecular breeding from monocotyledonous, dicotyledonous, and gymnosperm plants.
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