ArticleScientific reports2019
Development of a qPCR platform for quantification of the five bacteriophages within bacteriophage cocktail 2 (BFC2).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Design of M13 phage systems dual-displaying two functional proteins for developing a rapid biosensing probe.Journal of nanobiotechnology · 2026Article
- Robust detection in faeces andVeterinary and animal science · 2026Article
- A qPCR-Based Screening Platform for Exploratory Assessment of Phage Training Outcomes inViruses · 2026Article
- Translational Quantitative Proteomic Assay for Bacteriophages: A New Frontier in Phage Pharmaceutical Development.bioRxiv : the preprint server for biology · 2026Article
- Article
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- Latest Advances in Inhalable Dry Powder Bacteriophage Therapy for Pulmonary Infections.Pharmaceutics · 2025Review
- Evaluating phage lytic activity: from plaque assays to single-cell technologies.Frontiers in microbiology · 2025Review
- Phage-Mediated Digestive Decolonization in a Gut-On-A-Chip Model: A Tale of Gut-Specific Bacterial Prosperity.Viruses · 2024Article
- Evaluation of the impact of repeated intravenous phage doses on mammalian host-phage interactions.Journal of virology · 2024Article
- Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.PloS one · 2024Article
- The host phylogeny determines viral infectivity and replication across Staphylococcus host species.PLoS pathogens · 2023Article
- EudragitPharmaceutics · 2023Article
- Development of TaqMan Real-Time PCR Protocols for Simultaneous Detection and Quantification of the Bacterial PathogenViruses · 2023Article
- Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into Multiple Gut Pathogens.ACS synthetic biology · 2023Article
- Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii.BMC infectious diseases · 2022Article
- Exploring the diversity of bacteriophage specific to Oenococcus oeni and Lactobacillus spp and their role in wine production.Applied microbiology and biotechnology · 2021Review
- Article
- Manufacturing Bacteriophages (Part 1 of 2): Cell Line Development, Upstream, and Downstream Considerations.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Manufacturing Bacteriophages (Part 2 of 2): Formulation, Analytics and Quality Control Considerations.Pharmaceuticals (Basel, Switzerland) · 2021Review
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13 authors.
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Abstract
To determine phage titers accurately, reproducibly and in a non-laborious and cost-effective manner, we describe the development of a qPCR platform for molecular quantification of five phages present in bacteriophage cocktail 2 (BFC2). We compared the performance of this molecular approach, with regard to quantification and reproducibility, with the standard culture-based double agar overlay method (DAO). We demonstrated that quantification of each of the five phages in BFC2 was possible by means of qPCR, without prior DNA extraction, but yields were significantly higher in comparison to DAO. Although DAO is assumed to provide an indication of the number of infective phage particles, whereas qPCR only provides information on the number of phage genomes, the difference in yield (qPCR/DAO ratio) was observed to be phage-dependent and appeared rather constant for all phages when analyzing different (freshly prepared) stocks of these phages. While DAO is necessary to determine sensitivity of clinical strains against phages in clinical applications, qPCR might be a valid alternative for rapid and reproducible quantification of freshly prepared stocks, after initial establishment of a correction factor towards DAO.
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