ArticleScientific reports2019
A mutation in the methionine aminopeptidase gene provides phage resistance in Streptococcus thermophilus.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Type III CRISPR-Cas systems preferentially acquire spacers from early-expressed phage genes in a transcription-dependent manner.Nucleic acids research · 2026Article
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- Phage resistance mutations in a marine bacterium impact biogeochemically relevant cellular processes.Nature microbiology · 2026Article
- Structural basis for Salmonella infection by two Microviridae phages.Communications biology · 2025Article
- Strengthening phage resistance of Streptococcus thermophilus by leveraging complementary defense systems.Nature communications · 2025Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance toMicrobiology (Reading, England) · 2025Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance tobioRxiv : the preprint server for biology · 2025Article
- Antibiotic Resistance Gene Expression in Veterinary Probiotics: Two Sides of the Coin.Veterinary sciences · 2025Review
- Mini review advantages and limitations of lytic phages compared with chemical antibiotics to combat bacterial infections.Heliyon · 2024Review
- Isolation and characterization of bacteriophages specific toFrontiers in microbiology · 2024Article
- Investigation of CRISPR-Independent Phage Resistance Mechanisms Reveals a Role for FtsH in Phage Adsorption to Streptococcus thermophilus.Journal of bacteriology · 2023Article
- Mechanisms and clinical importance of bacteriophage resistance.FEMS microbiology reviews · 2022Review
- Efficacy ofViruses · 2021Article
- Article
- It is unclear how important CRISPR-Cas systems are for protecting natural populations of bacteria against infections by mobile genetic elements.Proceedings of the National Academy of Sciences of the United States of America · 2020Review
- Characterization of a Type II-A CRISPR-Cas System inmSphere · 2020Article
- Novel Genus of Phages Infecting Streptococcus thermophilus: Genomic and Morphological Characterization.Applied and environmental microbiology · 2020Article
- Therapeutic and biotechnological applications of substrate specific microbial aminopeptidases.Applied microbiology and biotechnology · 2020Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus thermophilus is a lactic acid bacterium widely used by the dairy industry for the manufacture of yogurt and specialty cheeses. It is also a Gram-positive bacterial model to study phage-host interactions. CRISPR-Cas systems are one of the most prevalent phage resistance mechanisms in S. thermophilus. Little information is available about other host factors involved in phage replication in this food-grade streptococcal species. We used the model strain S. thermophilus SMQ-301 and its virulent phage DT1, harboring the anti-CRISPR protein AcrIIA6, to show that a host gene coding for a methionine aminopeptidase (metAP) is necessary for phage DT1 to complete its lytic cycle. A single mutation in metAP provides S. thermophilus SMQ-301 with strong resistance against phage DT1. The mutation impedes a late step of the lytic cycle since phage adsorption, DNA replication, and protein expression were not affected. When the mutated strain was complemented with the wild-type version of the gene, the phage sensitivity phenotype was restored. When this mutation was introduced into other S. thermophilus strains it provided resistance against cos-type (Sfi21dt1virus genus) phages but replication of pac-type (Sfi11virus genus) phages was not affected. The mutation in the gene coding for the MetAP induces amino acid change in a catalytic domain conserved across many bacterial species. Introducing the same mutation in Streptococcus mutans also provided a phage resistance phenotype, suggesting the wide-ranging importance of the host methionine aminopeptidase in phage replication.
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