ArticleScientific reports2019
Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Recent Advances in Cryopreservation of Animal Genetic Resources: Principles, Influencing Factors, and Material-Specific Challenges.Animals : an open access journal from MDPI · 2026Review
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Conservation of Teleost Fishes: Current Status and the Role of Reproductive Technologies.Advances in experimental medicine and biology · 2026Review
- Multi-Omics Investigation into Why Viable Oogonial Stem Cells Can Still Be Isolated and Cultured from Post-MortemInternational journal of molecular sciences · 2025Article
- Post-thaw quality assessment of testicular fragments as a source of spermatogonial cells for surrogate production in the flatfish Solea senegalensis.Fish physiology and biochemistry · 2024Article
- Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost.BMC biology · 2024Article
- High species homology potentiates quantitative inflammation profiling in zebrafish using immunofluorescence.Heliyon · 2024Article
- Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation.Communications biology · 2023Article
- Spermatogonia From Cryopreserved Testes of Critically Endangered Chinese Sturgeon Efficiently Colonized and Preferentially Proliferated in the Recipient Gonads of Yangtze Sturgeon.Marine biotechnology (New York, N.Y.) · 2022Article
- Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture-A Practical Review.Animals : an open access journal from MDPI · 2022Review
- Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges.Animals : an open access journal from MDPI · 2022Review
- Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri.Scientific reports · 2021Article
- Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating.Nature communications · 2021Article
- Production of Chinese rosy bitterling offspring derived from frozen and vitrified whole testis by spermatogonial transplantation.Fish physiology and biochemistry · 2020Article
- MeagreAnimals : an open access journal from MDPI · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zebrafish is one of the most commonly used model organisms in biomedical, developmental and genetic research. The production of several thousands of transgenic lines is leading to difficulties in maintaining valuable genetic resources as cryopreservation protocols for eggs and embryos are not yet developed. In this study, we utilized testis cryopreservation (through both slow-rate freezing and vitrification) and spermatogonia transplantation as effective methods for long-term storage and line reconstitution in zebrafish. During freezing, utilization of 1.3 M of dimethyl sulfoxide (Me
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Registered trials
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