ArticleInternational journal of molecular sciences2019
Effect of Saliva Collection Methods on the Detection of Periodontium-Related Genetic and Epigenetic Biomarkers-A Pilot Study.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- The Impacts of Ramadan Intermittent Fasting on Saliva Flow-Rate and Metabolic Data: A Systematic Review.Frontiers in nutrition · 2022Pooled it
- Detection of Salivary Small Extracellular Vesicles Associated Inflammatory Cytokines Gene Methylation in Gingivitis.International journal of molecular sciences · 2020Trial
- Impact of smoking and periodontitis on salivary IL-39, IL-41, IL-1β, and TNF-α levels: a cross-sectional analysis.BMC oral health · 2026Article
- The Salivary Transcriptome: A Window into Local and Systemic Gene Expression Patterns.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Microparticles Mediate Lipopolysaccharide-induced Inflammation and Chronic Pain in Mouse Model.Neuromolecular medicine · 2024Article
- Promising applications of human-derived saliva biomarker testing in clinical diagnostics.International journal of oral science · 2023Review
- Bioprinting extracellular vesicles as a "cell-free" regenerative medicine approach.Extracellular vesicles and circulating nucleic acids · 2023Review
- The role of extracellular vesicles in periodontitis: pathogenesis, diagnosis, and therapy.Frontiers in immunology · 2023Review
- The Relevance of DNA Methylation and Histone Modification in Periodontitis: A Scoping Review.Cells · 2022Article
- Salivary SARS-CoV-2 antibody detection using S1-RBD protein-immobilized 3D melt electrowritten poly(ε-caprolactone) scaffolds.RSC advances · 2022Article
- Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion.Nanomaterials (Basel, Switzerland) · 2021Article
- Assessment of Unstimulated Whole Salivary Tumor Necrosis Factor Alpha (TNF-α) and Cellular Micronuclei Levels in Snuff (Naswar) Users and Non-Users for Early Diagnosis of Oral Squamous Cell Carcinoma.International journal of environmental research and public health · 2021Article
- Magnetic detection of albuminuria using hematite nanorods synthesized via chemical hydrothermal method.Iranian journal of basic medical sciences · 2021Article
- Salivary Outer Membrane Vesicles and DNA Methylation of Small Extracellular Vesicles as Biomarkers for Periodontal Status: A Pilot Study.International journal of molecular sciences · 2021Article
- Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis.BioMed research international · 2021Review
- Saliva-Friend and Foe in the COVID-19 Outbreak.Diagnostics (Basel, Switzerland) · 2020Review
- Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status-A Pilot Study.International journal of molecular sciences · 2020Article
- Assessment of salivary and gingival crevicular fluids IL6, IL-8, IL-12, and IL-17 levels in healthy subjects and patients with periodontal disease.Journal of oral and maxillofacial pathology : JOMFPArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Different collection methods may influence the ability to detect and quantify biomarker levels in saliva, particularly in the expression of DNA/RNA methylation regulators of several inflammations and tissue turnover markers. This pilot study recruited five participants and unstimulated saliva were collected by either spitting or drooling, and the relative preference for each method was evaluated using a visual analogue scale. Subsequently, total RNA, gDNA and proteins were isolated using the Trizol method. Thereafter, a systematic evaluation was carried out on the potential effects of different saliva collection methods on periodontium-associated genes, DNA/RNA epigenetic factors and periodontium-related DNA methylation levels. The quantity and quality of DNA and RNA were comparable from different collection methods. Periodontium-related genes, DNA/RNA methylation epigenetic factors and periodontium-associated DNA methylation could be detected in the saliva sample, with a similar expression for both methods. The methylation of tumour necrosis factor-alpha gene promoter from drooling method showed a significant positive correlation (TNF α, r = 0.9) with clinical parameter (bleeding on probing-BOP). In conclusion, the method of saliva collection has a minimal impact on detecting periodontium-related genetic and epigenetic regulators in saliva. The pilot data shows that TNF α methylation may be correlated with clinical parameters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.