ArticleThe New phytologist2020
Cell and chloroplast anatomical features are poorly estimated from 2D cross-sections.
Article in The New phytologist, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Technological advances in imaging and modelling of leaf structural traits: a review of heat stress in wheat.Journal of experimental botany · 2026Review
- Rho GTPase overexpression impacts leaf internal architecture and mesophyll conductance in Arabidopsis.The New phytologist · 2025Article
- Characterization of Grain Structure Using Micro-CT and Identification of Related Candidate Genes by QTL Mapping in Foxtail Millet.Plants (Basel, Switzerland) · 2025Article
- Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis.The New phytologist · 2025Article
- The application and development of electron microscopy for three-dimensional reconstruction in life science: a review.Cell and tissue research · 2024Review
- Unusual nuclear structures in male meiocytes of wild-type rye as revealed by volume microscopy.Annals of botany · 2023Article
- Elevated nocturnal respiratory rates in the mitochondria of CAM plants: current knowledge and unanswered questions.Annals of botany · 2023Article
- A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in CPlant methods · 2023Article
- Creating a virtual leaf.AoB PLANTS · 2023Review
- 3-D reconstruction of rice leaf tissue for proper estimation of surface area of mesophyll cells and chloroplasts facing intercellular airspaces from 2-D section images.Annals of botany · 2022Article
- Localized growth and remodelling drives spongy mesophyll morphogenesis.Journal of the Royal Society, Interface · 2022Article
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- Structural organization of the spongy mesophyll.The New phytologist · 2022Article
- Elastic and collapsible: current understanding of cell walls in succulent plants.Journal of experimental botany · 2022Review
- X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs.Plant physiology · 2022Article
- Electron microscopy for imaging organelles in plants and algae.Plant physiology · 2022Article
- Ploidy influences wheat mesophyll cell geometry, packing and leaf function.Plant direct · 2021Article
- Maximum COProceedings. Biological sciences · 2021Article
- Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging.Nature communications · 2021Article
- Digitally deconstructing leaves in 3D using X-ray microcomputed tomography and machine learning.Applications in plant sciences · 2020Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Leaf function is intimately related to the size, shape, abundance and position of cells and chloroplasts. Anatomy has long been assessed and quantified in two dimensions with 3D structure inferred from 2D micrographs. Serial block face scanning electron microscopy (SBF-SEM) was used to reconstruct 95 cells and 1173 chloroplasts from three wheat and nine chickpea leaves (three samples each from three chickpea genotypes). Wheat chloroplast volume was underestimated by 61% in mesophyll cells and 45% in bundle sheath cells from 2D micrographs, whereas chickpea mesophyll chloroplast volume was underestimated by 60% using simple geometrical models. Models of chickpea spongy and palisade cells both under- and overestimated surface area and volume by varying degrees. These models did not adequately capture irregular shapes such as flattening of chloroplasts or lobed spongy mesophyll cells. It is concluded that simple geometrical models to estimate chloroplast and cell 3D volume and surface area from 2D micrographs are inadequate, and that SBF-SEM has strong potential to contribute to improved understanding of leaf form and function.
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