ArticleChemistry (Weinheim an der Bergstrasse, Germany)2020
Computer-Assisted Recombination (CompassR) Teaches us How to Recombine Beneficial Substitutions from Directed Evolution Campaigns.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Recent advances on protein engineering for improved stability.Biodesign research · 2025Review
- Molecular Engineering L-Aspartate-Alpha-Decarboxylase to Enhance Catalytic Stability and Performance.ChemistryOpen · 2025Article
- Optimizing enzyme thermostability by combining multiple mutations using protein language model.mLife · 2024Article
- Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry.Foods (Basel, Switzerland) · 2024Review
- Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes.ACS catalysis · 2023Article
- A Competitive High-Throughput Screening Platform for Designing Polylactic Acid-Specific Binding Peptides.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A step forward to the optimized HlyA type 1 secretion system through directed evolution.Applied microbiology and biotechnology · 2023Article
- Identification and Mutation Analysis of Nonconserved Residues on the TIM-Barrel Surface of GH5_5 Cellulases for Catalytic Efficiency and Stability Improvement.Applied and environmental microbiology · 2022Article
- A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.Angewandte Chemie (International ed. in English) · 2022Article
- Polar Substitutions on the Surface of a Lipase Substantially Improve Tolerance in Organic Solvents.ChemSusChem · 2022Article
- Modulating the Coupling Efficiency of P450 BM3 by Controlling Water Diffusion through Access Tunnel Engineering.ChemSusChem · 2022Article
- In-depth Sequence-Function Characterization Reveals Multiple Pathways to Enhance Enzymatic Activity.ACS catalysis · 2022Article
- Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis.Computational and structural biotechnology journal · 2022Article
- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Sustainable isomaltulose production inFrontiers in microbiology · 2022Article
- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recombination of Compatible Substitutions by 2GenReP and InSiReP.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.Angewandte Chemie (International ed. in English) · 2021Article
- Tunnel engineering for modulating the substrate preference in cytochrome P450Bioresources and bioprocessing · 2021Article
- Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase.Computational and structural biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
A main remaining challenge in protein engineering is how to recombine beneficial substitutions. Systematic recombination studies show that poorly performing variants are usually obtained after recombination of 3 to 4 beneficial substitutions. This limits researchers in exploiting nature's potential in generating better enzymes. The Computer-assisted Recombination (CompassR) strategy provides a selection guide for beneficial substitutions that can be recombined to gradually improve enzyme performance by analysis of the relative free energy of folding (ΔΔG
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