Evidence map›Paper›PMID 31552297›Full record

ArticleCommunications biology2019

Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.

Siyu Feng, Aruna Varshney, Doris Coto Villa, Cyrus Modavi, John Kohler, Fatima Farah, Shuqin Zhou, Nebat Ali, Joachim D Müller, Miri K Van Hoven and 1 more

Abstract read
In one paragraph

Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed.

  1. Article
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  13. Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025
    Review
  14. Article
  15. Article
  16. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Siyu Feng1The UC Berkeley-UCSF Graduate Program in Bioengineering, San Francisco, CA 94143 USA.ORCID 0000-0001-8499-6914
Aruna Varshney2Department of Biological Sciences, San Jose State University, San Jose, CA 95192 USA.
Doris Coto Villa2Department of Biological Sciences, San Jose State University, San Jose, CA 95192 USA.
Cyrus Modavi3Department of Bioengineering and Therapeutic Sciences, University of California in San Francisco, San Francisco, CA 94143 USA.
John Kohler4School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 USA.
Fatima Farah2Department of Biological Sciences, San Jose State University, San Jose, CA 95192 USA.
Shuqin Zhou5School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084 China.
Nebat Ali2Department of Biological Sciences, San Jose State University, San Jose, CA 95192 USA.
Joachim D Müller4School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 USA.
Miri K Van Hoven2Department of Biological Sciences, San Jose State University, San Jose, CA 95192 USA.
Bo Huang6Department of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA 94143 USA.ORCID 0000-0003-1704-4141

Funding

NIGMS MARC UNDERGRADUATE RESEARCH TRAINING GRANTT34GM008253 · NIGMS · SAN JOSE STATE UNIVERSITY · PI OUVERNEY, CLEBER COSTA · 1988 to 2020
$8.4M
Olfactory memory acquisition consolidation and recallR01DC005991 · NIDCD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI L 'ETOILE, NOELLE D · 2005 to 2022
$7.1M
The Effects of Stimulation and Sleep on Neural Circuit ConnectionsR01NS087544 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Noelle D L 'Etoile, Miri Kerensa VanHoven · 2014 to 2026
$6.2M
In vivo Fluorescence Fluctuation Spectroscopy - Administrative SupplementR01GM064589 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI MUELLER, JOACHIM D · 2002 to 2019
$4.6M
Structure mapping of molecular complexes by super-resolution microscopyR01GM124334 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2018 to 2021
$1.3M
Molecular Mechanisms of Neural Circuit FormationSC3GM089595 · NIGMS · SAN JOSE STATE UNIVERSITY · PI VANHOVEN, MIRI KERENSA · 2010 to 2017
$837k
Rapid screening of gene-edited cellsR21GM129652 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2018 to 2019
$441k
Multimerized GFP probe for live cell imagingR21EB022798 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2016 to 2017
$436k
NIBIB NIH HHS R21 EB022798NIDCD NIH HHS R01 DC005991NIGMS NIH HHS R01 GM064589NIGMS NIH HHS R01 GM124334NIGMS NIH HHS R21 GM129652NIGMS NIH HHS SC3 GM089595NIGMS NIH HHS T34 GM008253NINDS NIH HHS R01 NS087544
6 · The paper itself

Abstract

Self-associating split fluorescent proteins (FPs) are split FPs whose two fragments spontaneously associate to form a functional FP. They have been widely used for labeling proteins, scaffolding protein assembly and detecting cell-cell contacts. Recently developments have expanded the palette of self-associating split FPs beyond the original split GFP

Indexed as

Gene ExpressionGenes, ReporterAmino Acid SequenceFluorescent DyesHEK293 CellsHumansLuminescent ProteinsMicroscopy, FluorescenceModels, MolecularMolecular ImagingRed Fluorescent ProteinStructure-Activity RelationshipSynapsesFluorescent DyesLuminescent ProteinsRed Fluorescent ProteinFluorescence imagingFluorescent proteinsGenetic engineeringNeural circuits

Identifiers

PMID31552297
PMCPMC6749000

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.