Evidence map›Paper›PMID 31551402›Full record

ArticlemSystems2019

Simultaneous Tracking of Pseudomonas aeruginosa Motility in Liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators.

Andrew L Hook, James L Flewellen, Jean-Frédéric Dubern, Alessandro M Carabelli, Irwin M Zaid, Richard M Berry, Ricky D Wildman, Noah Russell, Paul Williams, Morgan R Alexander

Open access · goldAbstract read
In one paragraph

Article in mSystems, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 35 citations in OpenAlex.

  1. Journal of bacteriology · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Species-specific surface dwell times and accumulation of microbes investigated by holographic microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2025
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. HowmBio · 2024
    Article
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  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Andrew L HookAdvanced Materials and Healthcare Technologies Division, School of Pharmacy, University of Nottingham, Nottingham, United Kingdom.ORCID https://orcid.org/0000-0001-7756-8444
James L FlewellenImmune Receptor Activation Laboratory, The Francis Crick Institute, London, United Kingdom.ORCID https://orcid.org/0000-0003-1889-5183
Jean-Frédéric DubernCentre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Alessandro M CarabelliAdvanced Materials and Healthcare Technologies Division, School of Pharmacy, University of Nottingham, Nottingham, United Kingdom.
Irwin M ZaidDepartment of Physics, Clarendon Laboratory, University of Oxford, Oxford, United Kingdom.
Richard M BerryDepartment of Physics, Clarendon Laboratory, University of Oxford, Oxford, United Kingdom.
Ricky D WildmanDepartment of Chemical and Environmental Engineering, School of Engineering, University of Nottingham, Nottingham, United Kingdom.
Noah RussellMarine Biological Association, The Laboratory, Plymouth, United Kingdom.
Paul WilliamsCentre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom paul.williams@nottingham.ac.uk morgan.alexander@nottingham.ac.uk.ORCID https://orcid.org/0000-0002-1920-5036
Morgan R AlexanderAdvanced Materials and Healthcare Technologies Division, School of Pharmacy, University of Nottingham, Nottingham, United Kingdom paul.williams@nottingham.ac.uk morgan.alexander@nottingham.ac.uk.ORCID https://orcid.org/0000-0001-5182-493X
University of Nottingham · GBUniversity of Oxford · GBMarine Biological Association of the United Kingdom · GBThe Francis Crick Institute · GB

Funding

Wellcome Trust 103882Wellcome Trust 103884
6 · The paper itself

Abstract

Bacteria sense chemicals, surfaces, and other cells and move toward some and away from others. Studying how single bacterial cells in a population move requires sophisticated tracking and imaging techniques. We have established quantitative methodology for label-free imaging and tracking of individual bacterial cells simultaneously within the bulk liquid and at solid-liquid interfaces by utilizing the imaging modes of digital holographic microscopy (DHM) in three dimensions (3D), differential interference contrast (DIC), and total internal reflectance microscopy (TIRM) in two dimensions (2D) combined with analysis protocols employing bespoke software. To exemplify and validate this methodology, we investigated the swimming behavior of a

Indexed as

3D imagingbiofilmsdigitial holographic microscopyflagellar motilitystators

Identifiers

PMID31551402
PMCPMC6759568
OpenAlexW2975880844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.