Evidence map›Paper›PMID 31551072›Full record

ArticleAnnals of clinical microbiology and antimicrobials2019

Detection and molecular characterization of urinary tract HIV-1 populations.

M L Mzingwane, G Hunt, R Lassauniere, M Kalimashe, A Bongwe, J Ledwaba, R E Chaisson, N Martinson, K Richter, S M Bowyer and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of clinical microbiology and antimicrobials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 4 countries.

M L MzingwaneDepartment of Pathology, Faculty of Medicine, National University of Science & Technology, Ascot, P. O Box AC 939, Bulawayo, Zimbabwe. mayibongwe.mzingwane@nust.ac.zw.ORCID http://orcid.org/0000-0002-7262-1901
G HuntCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
R LassauniereCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
M KalimasheCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
A BongweCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
J LedwabaCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
R E ChaissonJohns Hopkins University Center for AIDS Research, Baltimore, MD, USA.
N MartinsonPerinatal HIV Research Unit (PHRU), SA MRC Soweto Matlosana Collaborating Centre for HIV/AIDS and TB, University of the Witwatersrand, Johannesburg, South Africa.
K RichterDepartment of Medical Virology, University of Pretoria, Pretoria, South Africa.
S M BowyerDepartment of Medical Virology, University of Pretoria, Pretoria, South Africa.
C T TiemessenCentre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.
National Institute for Communicable DiseasesUniversity of the Witwatersrand · ZAJohns Hopkins University · USNational Health Laboratory Service · ZANational University of Science and Technology · ZWStatens Serum Institut · DKUniversity of Pretoria · ZA

Funding

Vaccine Response and Immunotherapeutics SWGP30AI094189 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Richard E. Chaisson · 2012 to 2026
$67.0M
Longitudinal Study of HIV-Associated Lung Infections in SowetoR01HL090312 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI CHAISSON, RICHARD E. · 2007 to 2012
$3.3M
National Institutes of Health, USA P30AI094189National Institutes of Health, USA R01HL090312NHLBI NIH HHS R01 HL090312NIAID NIH HHS P30 AI094189
6 · The paper itself

Abstract

backgroundIdentification of all possible HIV reservoirs is an important aspect in HIV eradication efforts. The urinary tract has however not been well studied as a potential HIV reservoir. In this pilot study we molecularly characterized HIV-1 viruses in urine and plasma samples to investigate HIV-1 replication, compartmentalization and persistence in the urinary tract.

methodsProspectively collected urine and blood samples collected over 12-36 months from 20 HIV-1 infected individuals were analysed including sampling points from prior to and after ART initiation. HIV-1 pol gene RNA and DNA from urine supernatant and urine pellets respectively were analysed and compared to plasma RNA viruses from the same individual.

resultsHIV-1 nucleic acid was detected in urine samples from at least one time point in 8/20 (40%) treatment-naïve subjects compared to 1/13 (7.7%) individuals on antiretroviral treatment (ART) during periods of plasma viral suppression and 1/7 (14.3%) individuals with virological failure. HIV-1 RNA was undetectable in urine samples after ART initiation but HIV-1 DNA was detectable in one patient more than 6 months after treatment initiation. There was co-clustering of urine-derived pol sequences but some urine-derived sequences were interspersed among the plasma-derived sequences.

conclusionsSuppressive ART reduces HIV-1 replication in the urinary tract but HIV-1 DNA may persist in these cells despite treatment. A larger number of sequences would be required to confirm HIV compartmentalization in the urinary tract.

Indexed as

GenotypeAdultAnti-Retroviral AgentsDNA, ViralFemaleHIV-1HIV InfectionsHumansMalePilot ProjectsPlasmapol Gene Products, Human Immunodeficiency VirusProspective StudiesRNA, ViralSequence Analysis, DNAUrinary TractAnti-Retroviral AgentsDNA, Viralpol Gene Products, Human Immunodeficiency VirusRNA, Viral

Identifiers

PMID31551072
PMCPMC6760049
OpenAlexW2975087048

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.