Evidence map›Paper›PMID 31550256›Full record

ArticlePloS one2019

De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress.

Xiaohua Du, Xiaopei Zhu, Yaping Yang, Yanli Wang, Paul Arens, Huichao Liu

Abstract read
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaohua DuSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.ORCID 0000-0001-8619-6419
Xiaopei ZhuSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Yaping YangSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Yanli WangSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Paul ArensWageningen University & Research, Wageningen, The Netherlands.
Huichao LiuSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bred, but the mechanisms of their heat resistance are not understood. In this study, we investigated the transcript profiles of a heat-tolerant pansy inbred line, DFM16, in response to high temperatures using RNAseq. Approximately 55.48 Gb of nucleotide data were obtained and assembled into 167,576 unigenes with an average length of 959 bp, of which, 5,708 genes were found to be differentially expressed after heat treatments. Real-time qPCR was performed to validate the expression profiles of the selected genes. Nine metabolic pathways were found to be significantly enriched, in the analysis of the differentially expressed genes. Several potentially interesting genes that encoded putative transcription regulators or key components involving heat shock protein (HSP), heat shock transcription factors (HSF), and antioxidants biosynthesis, were identified. These genes were highlighted to indicate their significance in response to heat stress and will be used as candidate genes to improve pansy heat-tolerance in the future.

Indexed as

Gene Expression ProfilingGene Expression Regulation, PlantGenes, PlantHeat-Shock ResponseHot TemperaturePhenotypeReal-Time Polymerase Chain ReactionSeedlingsTranscriptomeViola

Identifiers

PMID31550256
PMCPMC6759194

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.