ArticleMolecular microbiology2019
A PopZ-linked apical recruitment assay for studying protein-protein interactions in the bacterial cell envelope.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- The penicillin-binding protein PBP1b fortifies the Escherichia coli division site against osmotic rupture.Nature microbiology · 2026Article
- A potential role for acyl-phosphate in the coordination of phospholipid and lipopolysaccharide synthesis inbioRxiv : the preprint server for biology · 2026Article
- Structural modeling reveals the allosteric switch controlling the chitin utilization program ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae.PLoS genetics · 2025Article
- Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Light-dependent modulation of protein localization and function in living bacteria cells.Nature communications · 2024Article
- Molecular insights into the initiation step of the Rcs signaling pathway.Structure (London, England : 1993) · 2024Article
- Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium.Journal of bacteriology · 2024Article
- Protein-Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system.PLoS genetics · 2023Article
- The divisome but not the elongasome organizes capsule synthesis in Streptococcus pneumoniae.Nature communications · 2023Article
- The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.PLoS genetics · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- FrzS acts as a polar beacon to recruit SgmX, a central activator of type IV pili during Myxococcus xanthus motility.The EMBO journal · 2023Article
- FtsEX-independent control of RipA-mediated cell separation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Suppressor Mutants: History and Today's Applications.EcoSal Plus · 2021Review
- Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications.Nature methods · 2021Article
- Construction of intracellular asymmetry and asymmetric division in Escherichia coli.Nature communications · 2021Article
- Intrinsically Disordered Bacterial Polar Organizing Protein Z, PopZ, Interacts with Protein Binding Partners Through an N-terminal Molecular Recognition Feature.Journal of molecular biology · 2020Article
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2 authors.
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Abstract
Most bacteria are surrounded by a complex cell envelope. As with many biological processes, studies of envelope assembly have benefited from cell-based assays for detecting protein-protein interactions. These assays use simple readouts and lack a protein purification requirement, making them ideal for early stage investigations. The most widely used two-hybrid interaction assay for proteins involved in envelope biogenesis is based on the reconstitution of adenylate cyclase activity from a split enzyme. Because adenylate cyclase is only functional in the cytoplasm, both protein fusions used in the assay must have a terminus located in this compartment. However, many envelope assembly factors are wholly extracytoplasmic. Detecting interactions involving such proteins using two-hybrid systems has therefore been problematic. To address this issue, we developed a cytological assay in Escherichia coli based on PopZ from Caulobacter crescentus. Here, we demonstrate the utility of this PopZ-Linked Apical Recruitment (POLAR) method for detecting interactions between proteins located in different cellular compartments. Additionally, we report that recruitment of an active peptidoglycan synthase to the cell pole is detrimental for E. coli and that interactions between proteins in the inner and outer membranes of the Gram-negative envelope may provide a mechanism for recruiting protein complexes to subpolar sites.
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