Evidence map›Paper›PMID 31550045›Full record

ArticleMolecular biology and evolution2020

The Universally Conserved Residues Are Not Universally Required for Stable Protein Expression or Functions of Cryptochromes.

Huachun Liu, Tiantian Su, Wenjin He, Qin Wang, Chentao Lin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology and evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. A structural view of plant CRY2 photoactivation and inactivation.Nature structural & molecular biology · 2020
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Huachun LiuDepartment of Molecular, Cell & Developmental Biology, University of California, Los Angeles, Los Angeles, CA.
Tiantian SuDepartment of Molecular, Cell & Developmental Biology, University of California, Los Angeles, Los Angeles, CA.
Wenjin HeDepartment of Molecular, Cell & Developmental Biology, University of California, Los Angeles, Los Angeles, CA.
Qin WangUCLA-FAFU Joint Research Center on Plant Proteomics, Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou, China.
Chentao LinDepartment of Molecular, Cell & Developmental Biology, University of California, Los Angeles, Los Angeles, CA.
University of California, Los Angeles · USFujian Agriculture and Forestry University · CNFujian Normal University · CN

Funding

The signaling mechanisms of Arabidopsis CRY2R01GM056265 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LIN, CHENTAO · 2001 to 2021
$9.5M
PLANT PHOTOSENSORY RECEPTOR CRY2--SIGNALING MECHANISMR29GM056265 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LIN, CHENTAO · 1997 to 2001
$204k
NIGMS NIH HHS R01 GM056265NIGMS NIH HHS R29 GM056265
6 · The paper itself

Abstract

Universally conserved residues (UCRs) are invariable amino acids evolutionarily conserved among members of a protein family across diverse kingdoms of life. UCRs are considered important for stability and/or function of protein families, but it has not been experimentally examined systematically. Cryptochromes are photoreceptors in plants or light-independent components of the circadian clocks in mammals. We experimentally analyzed 51 UCRs of Arabidopsis cryptochrome 2 (CRY2) that are universally conserved in eukaryotic cryptochromes from Arabidopsis to human. Surprisingly, we found that UCRs required for stable protein expression of CRY2 in plants are not similarly required for stable protein expression of human hCRY1 in human cells. Moreover, 74% of the stably expressed CRY2 proteins mutated in UCRs retained wild-type-like activities for at least one photoresponses analyzed. Our finding suggests that the evolutionary mechanisms underlying conservation of UCRs or that distinguish UCRs from non-UCRs determining the same functions of individual cryptochromes remain to be investigated.

Indexed as

Amino Acid SequenceArabidopsisArabidopsis ProteinsConserved SequenceCryptochromesEvolution, MolecularGene Expression Regulation, PlantHEK293 CellsHumansModels, MolecularMutationProtein ConformationProtein StabilityArabidopsis ProteinsCRY1 protein, humanCRY2 protein, ArabidopsisCryptochromesArabidopsisblue-light receptorcryptochromeuniversally conserved residues

Identifiers

PMID31550045
PMCPMC8204711
OpenAlexW2975140466

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.