ArticlePoultry science2019
Evaluation of in ovo Bacillus spp. based probiotic administration on horizontal transmission of virulent Escherichia coli in neonatal broiler chickens.
Article in Poultry science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Probiotics in Poultry: A Comprehensive Review of Mechanisms, Applications, and Future Directions.Veterinary sciences · 2026Review
- Comparative efficacy of oral and cloacal administration of Lactobacillus probiotics and postbiotics against Campylobacter jejuni colonization in broiler chickens.Poultry science · 2026Article
- In vitro compatibility screening of Bacillus pumilus and Bacillus altitudinis strains with selected candidate prebiotics for in ovo application of synergistic synbiotics.Applied microbiology and biotechnology · 2025Article
- Probiotic application to hatching egg surface supports microbiota development and acquisition in broiler embryos and hatchlings.Poultry science · 2025Article
- Article
- Bacillus safensis M01 reversed the inflammatory injury of mice jejunum caused by enterotoxigenic Escherichia coli K88.Archives of microbiology · 2025Article
- Effect of In Ovo Supplementation of Slab51 Probiotic Mixture, Associated with Marek's Disease Vaccine, on Growth Performance, Intestinal Morphology andAnimals : an open access journal from MDPI · 2024Article
- Article
- Control ofAntibiotics (Basel, Switzerland) · 2024Review
- Article
- Physiological effects ofFrontiers in veterinary science · 2023Review
- Article
- Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens.Animals : an open access journal from MDPI · 2021Review
- The Potential of Probiotics to Eradicate Gut Carriage of Pathogenic or Antimicrobial-ResistantAntibiotics (Basel, Switzerland) · 2021Review
- Review
- Live Bacterial Prophylactics in Modern Poultry.Frontiers in veterinary science · 2020Review
- Article
- Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluated the effect of in ovo Bacillus spp. base probiotic (BBP) administration on hatchability, Gram-negative bacteria (GNB) recovery, performance, and microbiota composition in 2 independent trials using a virulent E. coli seeder challenge model. In each trial, one hundred and eighty 18-day-old embryos were allocated into 1 of 2 groups: Control and treated group (inoculated with 107 BBP). On day 19 of embryogenesis, seeder embryos (n = 18) were inoculated with 4.5 × 104E. coli/mL+272 μg/mL tetracycline and segregated into mesh hatching bags. Twelve chicks per group were euthanized at hatch and at day 7 to evaluate the gastrointestinal composition of total GNB or total aerobic pasteurized bacteria. Also, in trial 2, ceca content from five chickens at day 7 were collected to evaluate microbiota composition. Embryos inoculated with BBP showed a significant (P < 0.05) reduction in the total number of GNB at day-of-hatch (DOH) and day 7. Probiotic treatment increased BW at DOH and day 7, and BW gain (days 0 to 7) when compared with Control chickens. Proteobacteria phylum was significantly reduced, while the Firmicutes was significantly increased by the BBP as compared to the Control (P < 0.05). At family level, Enterobacteriaceae was significantly decreased, while the Lachnospiraceae was significantly elevated in the BBP as compared to the Control group (P < 0.05). The genus Oscillospira was significantly enriched in the BBP group, whereas the unidentified genus of family Enterobacteriaceae in the Control group (P < 0.05). The BBP group increased the bacterial species richness, although there was no significant difference between treatments (P > 0.05). Interestingly, beta diversity showed a significant difference in bacterial community structure between Control and BBP groups (P < 0.05). The results of the present study suggest that in ovo administration of a BBP can reduce the severity of virulent E. coli horizontal transmission and infection of broiler chickens during hatch. The reduction in the severity of the transmission and infection by the BPP might be achieved through alterations of microbiota composition and its community structure.
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