ArticleScientific reports2019
Continuous photoproduction of hydrocarbon drop-in fuel by microbial cell factories.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Brown algae produce hydrocarbons via fatty acid photodecarboxylase-dependent and fatty acid photodecarboxylase-independent pathways.Plant physiology · 2026Article
- Integrating microbial cell factory with new-to-nature photobiocatalysis for de novo biosynthesis of D-homotryptophan.Nature communications · 2026Article
- On the Origin of the Red-Shifted Flavin Absorption Spectra in Fatty Acid Photodecarboxylase.The journal of physical chemistry. B · 2026Article
- Photoenzymatic Decarboxylation to Produce Hydrocarbon Fuels: A Critical Review.Molecular biotechnology · 2025Review
- Decarboxylation in Natural Products Biosynthesis.JACS Au · 2024Review
- The Prokaryotic MicroalgaPolish journal of microbiology · 2023Article
- Photobiocatalytic Strategies for Organic Synthesis.Chemical reviews · 2023Review
- Autocatalytic effect boosts the production of medium-chain hydrocarbons by fatty acid photodecarboxylase.Science advances · 2023Article
- Ultrafast Dynamics and Catalytic Mechanism of Fatty Acid Photodecarboxylase.Angewandte Chemie (International ed. in English) · 2022Article
- Microbial pathways for advanced biofuel production.Biochemical Society transactions · 2022Review
- Isobutene production inMetabolic engineering communications · 2021Article
- A Reconstructed Common Ancestor of the Fatty Acid Photo-decarboxylase Clade Shows Photo-decarboxylation Activity and Increased Thermostability.Chembiochem : a European journal of chemical biology · 2021Article
- Photochemical Mechanism of Light-Driven Fatty Acid Photodecarboxylase.ACS catalysis · 2020Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Use of microbes to produce liquid transportation fuels is not yet economically viable. A key point to reduce production costs is the design a cell factory that combines the continuous production of drop-in fuel molecules with the ability to recover products from the cell culture at low cost. Medium-chain hydrocarbons seem ideal targets because they can be produced from abundant fatty acids and, due to their volatility, can be easily collected in gas phase. However, pathways used to produce hydrocarbons from fatty acids require two steps, low efficient enzymes and/or complex electron donors. Recently, a new hydrocarbon-forming route involving a single enzyme called fatty acid photodecarboxylase (FAP) was discovered in microalgae. Here, we show that in illuminated E. coli cultures coexpression of FAP and a medium-chain fatty acid thioesterase results in continuous release of volatile hydrocarbons. Maximum hydrocarbon productivity was reached under low/medium light while higher irradiance resulted in decreased amounts of FAP. It was also found that the production rate of hydrocarbons was constant for at least 5 days and that 30% of total hydrocarbons could be collected in the gas phase of the culture. This work thus demonstrates that the photochemistry of the FAP can be harnessed to design a simple cell factory that continuously produces hydrocarbons easy to recover and in pure form.
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