Evidence map›Paper›PMID 31548362›Full record

ArticleBioscience reports2019

Silencing of SPARC represses heterotopic ossification via inhibition of the MAPK signaling pathway.

Qianjun Wang, Qianqian Yang, Ali Zhang, Zhiqiang Kang, Yingsheng Wang, Zhentao Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

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  7. Osteonectin bidirectionally regulates osteoblast mineralization.Journal of orthopaedic surgery and research · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Qianjun WangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Qianqian YangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Ali ZhangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Zhiqiang KangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Yingsheng WangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Zhentao ZhangDepartment of Orthopedics, The 89th Hospital of Chinese People's Liberation Army, Weifang 261021, P.R. China.
Chinese People's Liberation Army · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heterotopic ossification (HO), the pathologic formation of extraskeletal bone, can be disabling and lethal. However, the underlying molecular mechanisms were largely unknown. The present study aimed to clarify the involvement of secreted protein acidic and rich in cysteine (SPARC) and the underlying mechanism in rat model of HO. The mechanistic investigation on roles of SPARC in HO was examined through gain- and loss-of-function approaches of SPARC, with alkaline-phosphatase (ALP) activity, mineralized nodules, and osteocalcin (OCN) content measured. To further confirm the regulatory role of SPARC, levels of mitogen-activated protein kinase (MAPK) signaling pathways-related proteins (extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), p38, nuclear factor κ-B (NF-κB), and IkB kinase β (IKKβ)) were determined. Bone marrow mesenchymal stem cells were treated with pathway inhibitor to investigate the relationship among SPARC, MAPK signaling pathway, and HO. The results suggested that SPARC expression was up-regulated in Achilles tendon tissues of HO rats. Silencing of SPARC could decrease phosphorylation of ERK, JNK, p38, NF-κB, and IKKβ. Additionally, silencing of SPARC or inhibition of MAPK signaling pathway could reduce the ALP activity, the number of mineralized nodules, and OCN content, thus impeding HO. To sum up, our study identifies the inhibitory role of SPARC gene silencing in HO via the MAPK signaling pathway, suggesting SPARC presents a potential target for HO therapy.

Indexed as

AnimalsExtracellular Signal-Regulated MAP KinasesI-kappa B ProteinsJNK Mitogen-Activated Protein KinasesLIM Domain ProteinsMaleMAP Kinase Signaling SystemNF-kappa BOssification, HeterotopicOsteocalcinOsteonectinp38 Mitogen-Activated Protein KinasesPhosphorylationRatsRats, WistarSignal TransductionExtracellular Signal-Regulated MAP KinasesI kappa B beta proteinI-kappa B ProteinsJNK Mitogen-Activated Protein KinasesLIM Domain ProteinsNF-kappa BOsteocalcinOsteonectinp38 Mitogen-Activated Protein KinasesALP activityHeterotopic ossificationMineralized nodulesMitogen-activated protein kinase signaling pathwayOCN contentSecreted protein acidic and rich in cysteine

Identifiers

PMID31548362
PMCPMC6851515
OpenAlexW2975850903

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.