Evidence map›Paper›PMID 31547818›Full record

ArticleMalaria journal2019

Targeted Next Generation Sequencing for malaria research in Africa: current status and outlook.

Anita Ghansah, Edwin Kamau, Alfred Amambua-Ngwa, Deus S Ishengoma, Oumou Maiga-Ascofare, Lucas Amenga-Etego, Awa Deme, William Yavo, Milijaona Randrianarivelojosia, Plasmodium Diversity Network Africa and 5 more

Abstract read
In one paragraph

Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  4. The Portable Microhaplotype Object and Tools.bioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Anita GhansahNoguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, P. O. Box LG 581, Legon, Ghana.
Edwin KamauDepartment of Emerging and Infectious Diseases (DEID), United States Army Medical Research Directorate -Africa (USAMR D-A), Kenya Medical Research Institute (KEMRI), Kisumu, Kenya.
Alfred Amambua-NgwaParasite Molecular Biology, Disease Control and Elimination, Medical Research Council Unit The Gambia at LSHTM, Atlantic Road Fajara, Banjul, The Gambia.
Deus S IshengomaNational Institute for Medical Research (NIMR), Tanga, Tanzania.
Oumou Maiga-AscofareBernhard Nocht Institute for Topical Medicine (BNITM), Hamburg, Germany.
Lucas Amenga-EtegoWest Africa Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.
Awa DemeDepartment of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
William YavoFaculty of Pharmacy, Department of Parasitology and Mycology, Fe ́lix Houphoue ̈t-Boigny University, BPV 34, Abidjan, Côte d'Ivoire.
Milijaona RandrianarivelojosiaMalaria Research Unit, Institut Pasteur de Madagascar, Avaradoha, BP 1274, 101, Antananarivo, Madagascar.
Plasmodium Diversity Network Africa
Lynette Isabella Ochola-OyierKEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.
Gideon Kofi HelegbeDepartment of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, University for Development Studies, P. O. Box TL1883, Tamale, Northern Region, Ghana.
Jeffery BaileyWarren Alpert Medical School, Brown University, 55 Claverick St, Rm 314B, Providence, RI, 02903, USA.
Michael AlifrangisCentre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Abdoulaye DjimdeMalaria Research and Training Centre, University of Science, Techniques and Technologies of Bamako, Bamako, Mali. adjimde@icermali.org.ORCID http://orcid.org/0000-0003-0062-2283

Funding

Medical Research Council MC_EX_MR/K02440X/1Wellcome TrustWellcome Trust 107740/Z/15/Z
6 · The paper itself

Abstract

Targeted Next Generation Sequencing (TNGS) is an efficient and economical Next Generation Sequencing (NGS) platform and the preferred choice when specific genomic regions are of interest. So far, only institutions located in middle and high-income countries have developed and implemented the technology, however, the efficiency and cost savings, as opposed to more traditional sequencing methodologies (e.g. Sanger sequencing) make the approach potentially well suited for resource-constrained regions as well. In April 2018, scientists from the Plasmodium Diversity Network Africa (PDNA) and collaborators met during the 7th Pan African Multilateral Initiative of Malaria (MIM) conference held in Dakar, Senegal to explore the feasibility of applying TNGS to genetic studies and malaria surveillance in Africa. The group of scientists reviewed the current experience with TNGS platforms in sub-Saharan Africa (SSA) and identified potential roles the technology might play to accelerate malaria research, scientific discoveries and improved public health in SSA. Research funding, infrastructure and human resources were highlighted as challenges that will have to be mitigated to enable African scientists to drive the implementation of TNGS in SSA. Current roles of important stakeholders and strategies to strengthen existing networks to effectively harness this powerful technology for malaria research of public health importance were discussed.

Indexed as

MalariaAfrica South of the SaharaCongresses as TopicHigh-Throughput Nucleotide SequencingHumansPlasmodiumSenegalAfricaMalariaTargeted Next Generation Sequencing

Identifiers

PMID31547818
PMCPMC6757370

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.