ArticleInternational journal of molecular sciences2019
Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes.Frontiers in plant science · 2022Pooled it
- The 26S proteasome engages in priming explants with auxin plus cytokinin for somatic embryogenesis of Coffea canephora.Protoplasma · 2026Article
- Integrated metabolomics, transcriptional, and physicochemical analysis reveals key metabolites and genes associated with somatic embryogenesis in Phyllostachys pubescens.Scientific reports · 2026Article
- Stage-specific metabolic divergence in flavonoid biosynthesis correlates with embryogenic capacity in rubber tree (Frontiers in plant science · 2026Article
- Optimization of Somatic Embryogenesis and Transcriptomic Analysis of the Early Stage of Callus Redifferentiation inPlants (Basel, Switzerland) · 2025Article
- Article
- Transcriptional Dynamics Underlying Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- The Identification and Characterization of WOX Family Genes inInternational journal of molecular sciences · 2024Article
- Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.Plant molecular biology · 2024Article
- Integrated metabolomics and transcriptomics analysis highlight key pathways involved in the somatic embryogenesis of Darjeeling tea.BMC genomics · 2024Article
- Article
- An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Primary Metabolite Screening Shows Significant Differences between Embryogenic and Non-Embryogenic Callus of Tamarillo (Plants (Basel, Switzerland) · 2023Article
- Transcriptomic and Metabolomic Investigation on Leaf Necrosis Induced byInternational journal of molecular sciences · 2023Article
- Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore.International journal of molecular sciences · 2023Article
- Dynamics of Endogenous Auxin and Its Role in Somatic Embryogenesis Induction and Progression in Cork Oak.Plants (Basel, Switzerland) · 2023Article
- Global transcriptome profiling reveals differential regulatory, metabolic and hormonal networks during somatic embryogenesis in Coffea arabica.BMC genomics · 2023Article
- Endogenous auxin maintains embryonic cell identity and promotes somatic embryo development in Arabidopsis.The Plant journal : for cell and molecular biology · 2023Article
- Plant Cell and Organism Development.International journal of molecular sciences · 2020Article
- Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
18 authors at 5 institutions in 2 countries.
Funding
Abstract
Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination of elite varieties. However, for many plant species, optimizing SE protocols still relies on a trial-and-error approach. Using coffee as a model plant, we report here the first global analysis of metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate and totipotency. Sampling from leaf explant dedifferentiation until embryo development covered 15 key stages. An in-depth statistical analysis performed on 104 metabolites revealed that massive re-configuration of metabolic pathways induced SE. During initial dedifferentiation, a sharp decrease in phenolic compounds and caffeine levels was also observed while auxins, cytokinins and ethylene levels were at their highest. Totipotency reached its highest expression during the callus stages when a shut-off in hormonal and metabolic pathways related to sugar and energetic substance hydrolysis was evidenced. Abscisic acid, leucine, maltotriose, myo-inositol, proline, tricarboxylic acid cycle metabolites and zeatin appeared as key metabolic markers of the embryogenic capacity. Combining metabolomics with multiphoton microscopy led to the identification of chlorogenic acids as markers of embryo redifferentiation. The present analysis shows that metabolite fingerprints are signatures of cell fate and represent a starting point for optimizing SE protocols in a rational way.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.