ArticleMetabolites2019
Normalizing Untargeted Periconceptional Urinary Metabolomics Data: A Comparison of Approaches.
Article in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Stability of Nε-Carboxymethyllysine and Nε-Carboxyethyllysine in Canine Urine Under Extended Room Temperature Storage.Animals : an open access journal from MDPI · 2026Article
- Food and Mood: Current Evidence on Mental Health and the Microbiota-Gut-Brain Axis.Current psychiatry reports · 2025Review
- Early changes in urineMetabolomics : Official journal of the Metabolomic Society · 2025Article
- Microflow Liquid Chromatography Coupled to Multinozzle Electrospray Ionization for Improved Lipidomics Coverage of 3D Clear Cell Renal Cell Carcinoma.Analytical chemistry · 2025Article
- Supplementation of Oocytes by Microinjection with Extra Copies of mtDNA Alters Metabolite Profiles and Interactions with Expressed Genes in a Tissue-Specific Manner.Biomolecules · 2024Article
- Propionic Acidemia, Methylmalonic Acidemia, and Cobalamin C Deficiency: Comparison of Untargeted Metabolomic Profiles.Metabolites · 2024Article
- Urinary 1-hydroxypyrene in pregnant women in a Northeastern U.S. city: socioeconomic disparity and contributions from air pollution sources.Journal of exposure science & environmental epidemiology · 2024Article
- Rapid discrimination of four Salmonella enterica serovars: A performance comparison between benchtop and handheld Raman spectrometers.Journal of cellular and molecular medicine · 2024Article
- Vitamin DEuropean journal of nutrition · 2024Article
- Comprehensive plasma steroidomics reveals subtle alterations of systemic steroid profile in patients at different stages of prostate cancer disease.Scientific reports · 2024Article
- Urinary Paraben Concentrations and Associations with the Periconceptional Urinary Metabolome: Untargeted and Targeted Metabolomics Analyses of Participants from the Early Pregnancy Study.Environmental health perspectives · 2023Article
- Metabolomic profiling to identify early urinary biomarkers and metabolic pathway alterations in autosomal dominant polycystic kidney disease.American journal of physiology. Renal physiology · 2023Article
- Giardia hinders growth by disrupting nutrient metabolism independent of inflammatory enteropathy.Nature communications · 2023Article
- Candida expansion in the gut of lung cancer patients associates with an ecological signature that supports growth under dysbiotic conditions.Nature communications · 2023Article
- A Complete Pipeline for Untargeted Urinary Volatolomic Profiling with Sorptive Extraction and Dual Polar and Nonpolar Column Methodologies Coupled with Gas Chromatography Time-of-Flight Mass Spectrometry.Analytical chemistry · 2023Article
- Article
- The untargeted urine volatilome for biomedical applications: methodology and volatilome database.Biological procedures online · 2022Review
- Evaluation of Normalization Approaches for Quantitative Analysis of Bile Acids in Human Feces.Metabolites · 2022Article
- NMR-Based Metabolomic Analysis of Sera in Mouse Models of CVB3-Induced Viral Myocarditis and Dilated Cardiomyopathy.Biomolecules · 2022Article
- Design and Methods of the Validating Injury to the Renal Transplant Using Urinary Signatures (VIRTUUS) Study in Children.Transplantation direct · 2021Article
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Authors and funding
7 authors.
Funding
Abstract
Metabolomics studies of the early-life exposome often use maternal urine specimens to investigate critical developmental windows, including the periconceptional period and early pregnancy. During these windows changes in kidney function can impact urine concentration. This makes accounting for differential urinary dilution across samples challenging. Because there is no consensus on the ideal normalization approach for urinary metabolomics data, this study's objective was to determine the optimal post-analytical normalization approach for untargeted metabolomics analysis from a periconceptional cohort of 45 women. Urine samples consisted of 90 paired pre- and post-implantation samples. After untargeted mass spectrometry-based metabolomics analysis, we systematically compared the performance of three common approaches to adjust for urinary dilution-creatinine adjustment, specific gravity adjustment, and probabilistic quotient normalization (PQN)-using unsupervised principal components analysis, relative standard deviation (RSD) of pooled quality control samples, and orthogonal partial least-squares discriminant analysis (OPLS-DA). Results showed that creatinine adjustment is not a reliable approach to normalize urinary periconceptional metabolomics data. Either specific gravity or PQN are more reliable methods to adjust for urinary concentration, with tighter quality control sample clustering, lower RSD, and better OPLS-DA performance compared to creatinine adjustment. These findings have implications for metabolomics analyses on urine samples taken around the time of conception and in contexts where kidney function may be altered.
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