ReviewAntibodies (Basel, Switzerland)2019
Using Nanobodies to Study Protein Function in Developing Organisms.
Review in Antibodies (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- NanoTag is an improved method to map interactions between DNA and proteins not requiring IgG.Scientific reports · 2025Article
- Atg8/LC3 controls systemic nutrient surplus signaling in flies and humans.Current biology : CB · 2024Article
- A β-catenin chromobody-based probe highlights endothelial maturation during vascular morphogenesis in vivo.Development (Cambridge, England) · 2024Article
- Extreme thermal stability of the antiGFP nanobody - GFP complex.BMC research notes · 2023Article
- Heterodimerization-dependent secretion of bone morphogenetic proteins in Drosophila.Developmental cell · 2023Article
- Article
- Engineered kinases as a tool for phosphorylation of selected targets in vivo.The Journal of cell biology · 2022Article
- Protein visualization and manipulation ineLife · 2022Article
- Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc.Nature communications · 2021Article
- Perturbation of kinetochore function using GFP-binding protein in fission yeast.G3 (Bethesda, Md.) · 2021Article
- Discovering signaling mechanisms governing metabolism and metabolic diseases with Drosophila.Cell metabolism · 2021Review
- A guide to: generation and design of nanobodies.The FEBS journal · 2021Review
- Protein manipulation using single copies of short peptide tags in cultured cells and inDevelopment (Cambridge, England) · 2021Article
- Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins.iScience · 2021Article
- Recombinant expression of nanobodies and nanobody-derived immunoreagents.Protein expression and purification · 2020Review
- Special Issue: Nanobody.Antibodies (Basel, Switzerland) · 2020Article
- Reflections on the use of protein binders to study protein function in developmental biology.Wiley interdisciplinary reviews. Developmental biology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyclonal and monoclonal antibodies have been invaluable tools to study proteins over the past decades. While indispensable for most biological studies including developmental biology, antibodies have been used mostly in fixed tissues or as binding reagents in the extracellular milieu. For functional studies and for clinical applications, antibodies have been functionalized by covalently fusing them to heterologous partners (i.e., chemicals, proteins or other moieties). Such functionalized antibodies have been less widely used in developmental biology studies. In the past few years, the discovery and application of small functional binding fragments derived from single-chain antibodies, so-called nanobodies, has resulted in novel approaches to study proteins during the development of multicellular animals in vivo. Expression of functionalized nanobody fusions from integrated transgenes allows manipulating proteins of interest in the extracellular and the intracellular milieu in a tissue- and time-dependent manner in an unprecedented manner. Here, we describe how nanobodies have been used in the field of developmental biology and look into the future to imagine how else nanobody-based reagents could be further developed to study the proteome in living organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.