Evidence map›Paper›PMID 31543471›Full record

ArticleStem cell reports2019

Single-Cell RNA Sequencing of hESC-Derived 3D Retinal Organoids Reveals Novel Genes Regulating RPC Commitment in Early Human Retinogenesis.

Xiying Mao, Qin An, Huiyu Xi, Xian-Jie Yang, Xiangmei Zhang, Songtao Yuan, Jinmei Wang, Youjin Hu, Qinghuai Liu, Guoping Fan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 66 citations in OpenAlex.

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  18. Retinal organoids as models for development and diseases.Cell regeneration (London, England) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Xiying MaoDepartment of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Qin AnDepartment of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, 695 Charles Young Drive South, Los Angeles, CA 90095, USA.
Huiyu XiDepartment of Ophthalmology, Xuzhou First People's Hospital of Xuzhou Medical University, Xuzhou Eye Research Institute, Xuzhou 221002, China.
Xian-Jie YangStein Eye Institute, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
Xiangmei ZhangStein Eye Institute, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
Songtao YuanDepartment of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Jinmei WangInstitute of Regenerative Medicine and International Lab of Ocular Stem Cells at Shanghai East Hospital, School of Life Science &Technology, Tongji University, Shanghai 200092, China.
Youjin HuState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. Electronic address: huyoujin@gzzoc.com.
Qinghuai LiuDepartment of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address: liuqh@njmu.edu.cn.
Guoping FanDepartment of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, 695 Charles Young Drive South, Los Angeles, CA 90095, USA. Electronic address: gfan@mednet.ucla.edu.
University of California, Los Angeles · USNanjing Medical University · CNJiangsu Province Hospital · CNShanghai East Hospital · CNSun Yat-sen University · CNXuzhou No.1 People's Hospital · CN

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Neuroprotection Mechanism for PhotoreceptorsR01EY026319 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YANG, XIAN-JIE · 2016 to 2025
$4.1M
Dissecting molecular pathways underlying Human Overgrowth SyndromeR01DE025474 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FAN, GUOPING · 2016 to 2020
$1.9M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY026319NIDCR NIH HHS R01 DE025474
6 · The paper itself

Abstract

The development of the mammalian retina is a complicated process involving the generation of distinct types of neurons from retinal progenitor cells (RPCs) in a spatiotemporal-specific manner. The progression of RPCs during retinogenesis includes RPC proliferation, cell-fate commitment, and specific neuronal differentiation. In this study, by performing single-cell RNA sequencing of cells isolated from human embryonic stem cell (hESC)-derived 3D retinal organoids, we successfully deconstructed the temporal progression of RPCs during early human retinogenesis. We identified two distinctive subtypes of RPCs with unique molecular profiles, namely multipotent RPCs and neurogenic RPCs. We found that genes related to the Notch and Wnt signaling pathways, as well as chromatin remodeling, were dynamically regulated during RPC commitment. Interestingly, our analysis identified that CCND1, a G

Indexed as

Gene Expression Regulation, DevelopmentalOrganoidsBiomarkersFluorescent Antibody TechniqueGene Expression ProfilingGene Regulatory NetworksHigh-Throughput Nucleotide SequencingHuman Embryonic Stem CellsHumansImmunophenotypingOrganogenesisRetinaSingle-Cell AnalysisTissue Culture TechniquesBiomarkerscommitmentearly human retinal developmenthuman embryonic stem cellretinaretinal progenitorsingle-cell RNA-seq

Identifiers

PMID31543471
PMCPMC6829752
OpenAlexW2974853044

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.